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	Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation
	Zhou, Jun ; Lin, Jian ; Zhou, Cuihong ; Deng, Xiaoyan ; Xia, Bin
刊名	欧洲生化学会联合会快报
	2011
关键词	Red fluorescent protein DsRed DsRed-Express2 Turbo RFP Bcl-xL Cytotoxicity CELL-DEATH INHIBITOR DISCOSOMA VARIANTS FAMILY
DOI	10.1016/j.febslet.2011.02.013
英文摘要	Red fluorescent protein (RFP) DsRed and its variants are widely applied in live-cell imaging experiments. However, a major factor that restricts the application of DsRed is its cytotoxicity. Here, we report that DsRed and its variant DsRed-Express2 inhibit the expression of B-cell lymphoma-extra large (Bcl-xL) in HeLa cells by translational regulation. Over-expression of Bcl-xL can reduce the cytotoxicity of DsRed. Meanwhile, Turbo RFP, a mutant RFP from Entacmaea quadricolor, does not affect Bcl-xL expression, suggesting that cytotoxic mechanisms of RFP from different species may be varied. Our results reveal a possible mechanism for the cytotoxicity of DsRed, providing a potential strategy to improve the application of DsRed and its variants. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); 6; ARTICLE; 5; 821-827; 585
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/240463]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhou, Jun,Lin, Jian,Zhou, Cuihong,et al. Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation[J]. 欧洲生化学会联合会快报,2011.
APA	Zhou, Jun,Lin, Jian,Zhou, Cuihong,Deng, Xiaoyan,&Xia, Bin.(2011).Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation.欧洲生化学会联合会快报.
MLA	Zhou, Jun,et al."Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation".欧洲生化学会联合会快报 (2011).

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