The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass   of PYL Proteins

doi:10.1016/j.molcel.2011.05.011

CORC > 北京大学 > 生命科学学院

	The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins
	Hao, Qi ; Yin, Ping ; Li, Wenqi ; Wang, Li ; Yan, Chuangye ; Lin, Zhaohu ; Wu, Jim Zhen ; Wang, Jiawei ; Yan, S. Frank ; Yan, Nieng
刊名	分子细胞
	2011
关键词	ABSCISIC-ACID SIGNAL-TRANSDUCTION MECHANISM START PYRABACTIN RECEPTORS SOFTWARE BINDING PLANTS
DOI	10.1016/j.molcel.2011.05.011
英文摘要	PYR1/PYL/RCAR proteins (PYLs) are confirmed abscisic acid (ABA) receptors, which inhibit protein phosphatase 2C (PP2C) upon binding to ABA. Arabidopsis thaliana has 14 PYLs, yet their functional distinction remains unclear. Here, we report systematic biochemical characterization of PYLs. A subclass of PYLs, represented by PYL10, inhibited PP2C in the absence of any ligand. Crystal structures of PYL10, both in the free form and in the HAB1 (PP2C)-bound state, revealed the structural basis for its constitutive activity. Structural-guided biochemical analyses revealed that ABA-independent inhibition of PP2C requires the PYLs to exist in a monomeric state. In addition, the residues guarding the entrance to the ligand-binding pocket of these PYLs should be bulky and hydrophobic. Based on these principles, we were able to generate monomeric PYL2 variants that gained constitutive inhibitory effect on PP2Cs. These findings provide an important framework for understanding the complex regulation of ABA signaling by PYL proteins.; Biochemistry & Molecular Biology; Cell Biology; SCI(E); 42; ARTICLE; 5; 662-672; 42
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/239578]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hao, Qi,Yin, Ping,Li, Wenqi,et al. The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins[J]. 分子细胞,2011.
APA	Hao, Qi.,Yin, Ping.,Li, Wenqi.,Wang, Li.,Yan, Chuangye.,...&Yan, Nieng.(2011).The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins.分子细胞.
MLA	Hao, Qi,et al."The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins".分子细胞 (2011).