Overexpression of a partial fragment of the salt-responsive gene OsNUC1   enhances salt adaptation in transgenic Arabidopsis thaliana and rice   (Oryza sativa L.) during salt stress

doi:10.1016/j.plantsci.2013.08.013

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	Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress
	Sripinyowanich, Siriporn ; Chamnanmanoontham, Nontalee ; Udomchalothorn, Thanikarn ; Maneeprasopsuk, Somporn ; Santawee, Panudda ; Buaboocha, Teerapong ; Qu, Li-Jia ; Gu, Hongya ; Chadchawan, Supachitra
刊名	plant science
	2013
关键词	Salt stress resistance Nucleolin Rice RNA recognition motif Glycine-arginine-rich repeat RNA-BINDING PROTEINS NUCLEOLAR PROTEIN EXPRESSION TOLERANCE DOMAINS LOCALIZATION CDNA COLD RECOGNITION ENCODES
DOI	10.1016/j.plantsci.2013.08.013
英文摘要	The rice (Oryza sativa L.) nucleolin gene, OsNUC1, transcripts were expressed in rice leaves, flowers, seeds and roots but differentially expressed within and between two pairs of salt-sensitive and salt-resistant rice lines when subjected to salt stress. Salt-resistant lines exhibited higher OsNUC1 transcript expression levels than salt-sensitive lines during 0.5% (w/v) NaCl salt stress for 6 d. Two sizes of OsNUC1 full-length cDNA were found in the rice genome database and northern blot analysis confirmed their existence in rice tissues. The longer transcript (OsNUC1-L) putatively encodes for a protein with a serine rich N-terminal, RNA recognition motifs in the central domain and a glycine- and arginine-rich repeat in the C-terminal domain, while the shorter one (OsNUC1-S) putatively encodes for the similar protein without the N-terminus. Without salt stress, OsNUC1-L expressing Arabidopsis thaliana Atnuc1-L1 plants displayed a substantial but incomplete revertant phenotype, whereas OsNUC1-S expression only induced a weak effect. However, under 0.5% (w/v) NaCl salt stress they displayed a higher relative growth rate, longer root length and a lower H2O2 level than the wild type plants, suggesting a higher salt resistance. Moreover, they displayed elevated AtSOS1 and AtP5CS1 transcript levels. We propose that OsNUC1-S plays an important role in salt resistance during salt stress, a new role for nucleolin in plants. (C) 2013 Elsevier Ireland Ltd. All rights reserved.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); 4; ARTICLE; 67-78; 213
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/219364]
专题	生命科学学院
推荐引用方式 GB/T 7714	Sripinyowanich, Siriporn,Chamnanmanoontham, Nontalee,Udomchalothorn, Thanikarn,et al. Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress[J]. plant science,2013.
APA	Sripinyowanich, Siriporn.,Chamnanmanoontham, Nontalee.,Udomchalothorn, Thanikarn.,Maneeprasopsuk, Somporn.,Santawee, Panudda.,...&Chadchawan, Supachitra.(2013).Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress.plant science.
MLA	Sripinyowanich, Siriporn,et al."Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress".plant science (2013).