A monoclonal antibody selected for probing the folding of Staphylococcal   nuclease and its N-terminal fragments

doi:10.1006/bbrc.1997.8060

CORC > 北京大学 > 生命科学学院

	A monoclonal antibody selected for probing the folding of Staphylococcal nuclease and its N-terminal fragments
	Cheng, P ; Zhou, JM ; Guo, ZQ
刊名	生物化学与生物物理学研究通讯
	1998
关键词	Staphylococcal nuclease monoclonal antibody protein folding epitope TRYPTOPHAN SYNTHASE IN-VITRO CONFORMATION PROTEIN RIBOSOME PEPTIDE
DOI	10.1006/bbrc.1997.8060
英文摘要	Monoclonal antibody McAb2C9 against Staphylococcal nuclease (SNase R) and its N-terminal fragments was produced and characterized. It was observed that the intact enzyme SNase Il. and its seven fragments (SNR141, SNR135, SNR121, SNR110, SNR102, SNR79 and SNR52) differed in their interactions with McAb2C9. However, the fragments with weak immunoreactivity, such as SNR141 and SNR110, increased ability reacting with McAb2C9 in their partially unfolded state. It suggests that the differences of immunoreactivity among the fragments are due to diverse extent of the exposure of the specific epitope and the conformation of the peptide fragment. The monoclonal antibody McAb2C9 could be a useful probe to investigate the mechanism of folding of SNase R and its N-terminal fragments. (C) 1998 Academic Press.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 2; ARTICLE; 2; 556-560; 244
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/217119]
专题	生命科学学院
推荐引用方式 GB/T 7714	Cheng, P,Zhou, JM,Guo, ZQ. A monoclonal antibody selected for probing the folding of Staphylococcal nuclease and its N-terminal fragments[J]. 生物化学与生物物理学研究通讯,1998.
APA	Cheng, P,Zhou, JM,&Guo, ZQ.(1998).A monoclonal antibody selected for probing the folding of Staphylococcal nuclease and its N-terminal fragments.生物化学与生物物理学研究通讯.
MLA	Cheng, P,et al."A monoclonal antibody selected for probing the folding of Staphylococcal nuclease and its N-terminal fragments".生物化学与生物物理学研究通讯 (1998).