Generation of a monkey with MECP2 mutations by TALEN-based gene   targeting

doi:10.1007/s12264-014-1434-8

CORC > 北京大学 > 生命科学学院

	Generation of a monkey with MECP2 mutations by TALEN-based gene targeting
	Liu, Zhen ; Zhou, Xue ; Zhu, Ying ; Chen, Zhi-Fang ; Yu, Bin ; Wang, Yan ; Zhang, Chen-Chen ; Nie, Yan-Hong ; Sang, Xiao ; Cai, Yi-Jun ; Zhang, Yue-Fang ; Zhang, Chen ; Zhou, Wen-Hao ; Sun, Qiang ; Qiu, Zilong
刊名	神经科学通报
	2014
关键词	monkey TALEN MeCP2 Rett syndrome CPG-BINDING PROTEIN-2 ONE-STEP GENERATION CYNOMOLGUS MONKEY RETT-SYNDROME MICE CHROMATIN MOUSE
DOI	10.1007/s12264-014-1434-8
英文摘要	Gene editing in model organisms has provided critical insights into brain development and diseases. Here, we report the generation of a cynomolgus monkey (Macaca fascicularis) carrying MECP2 mutations using transcription activator-like effector nucleases (TALENs)-mediated gene targeting. After injecting TALENs mRNA into monkey zygotes achieved by in vitro fertilization and embryo transplantation into surrogate monkeys, we obtained one male newborn monkey with an MECP2 deletion caused by frameshifting mutation in various tissues. The monkey carrying the MECP2 mutation failed to survive after birth, due to either the toxicity of TALENs or the critical requirement of MECP2 for neural development. The level of MeCP2 protein was essentially depleted in the monkey's brain. This study demonstrates the feasibility of introducing genetic mutations in non-human primates by site-specific gene-editing methods.; Neurosciences; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 4; ARTICLE; qsun@ion.ac.cn; zqiu@ion.ac.cn; 3; 381-386; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/213180]
专题	生命科学学院心理与认知科学学院
推荐引用方式 GB/T 7714	Liu, Zhen,Zhou, Xue,Zhu, Ying,et al. Generation of a monkey with MECP2 mutations by TALEN-based gene targeting[J]. 神经科学通报,2014.
APA	Liu, Zhen.,Zhou, Xue.,Zhu, Ying.,Chen, Zhi-Fang.,Yu, Bin.,...&Qiu, Zilong.(2014).Generation of a monkey with MECP2 mutations by TALEN-based gene targeting.神经科学通报.
MLA	Liu, Zhen,et al."Generation of a monkey with MECP2 mutations by TALEN-based gene targeting".神经科学通报 (2014).