双光子及共聚焦激光扫描显微术研究天花粉蛋白对人绒癌细胞的作用机制

doi:10.3321/j.issn:1000-3282.2001.05.025

CORC > 北京大学 > 生命科学学院

	双光子及共聚焦激光扫描显微术研究天花粉蛋白对人绒癌细胞的作用机制; Studying the effect of trichosanthin on choriocarcinoma cells with both two-photon and confocal laser scanning microscopy
	张春阳 ; 贡宜萱 ; 马辉 ; 安成才 ; 陈瓞延
刊名	生物化学与生物物理进展
	2001
关键词	双光子激光扫描显微术共聚焦激光扫描显微术天花粉蛋白人绒癌细胞活性氧自由基钙 two-photon laser scanning microscopy confocal laser scanning microscopy trichosanthin choriocarcinoma cells reactive oxygen species calcium
DOI	10.3321/j.issn:1000-3282.2001.05.025
英文摘要	天花粉蛋白 (trichosanthin ,TCS)是从中草药栝楼根中提取的一种核糖体失活蛋白 ,具有抗肿瘤和抗HIV功能 .应用双光子及共聚焦激光扫描显微术结合特异性荧光探针Hoechst 3334 2、 2′,7′ 二氯荧光黄双乙酸酯(DCFH DA)、Indo 1和Fluo 3 AM ,首次同时观察了TCS诱导人绒癌细胞 (JAR细胞 )凋亡过程中活性氧自由基 (ROS)和细胞内钙离子浓度 ([Ca2 + ]i)的变化 ,实验结果表明TCS引起的 [Ca2 + ]i 升高和ROS形成参与了TCS诱导的JAR细胞凋亡 ,并且ROS形成和 [Ca2 + ]i 升高有关 .共聚焦激光扫描显微术...; Trichosanthin (TCS), a ribosome inactivating protein extracted from the root tuber of a traditional Chinese medicinal herb Trichosanthes kirilowii, possesses anti-tumor and anti-human immunodeficiency virus (HIV) activities. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, 2', 7'-dichlorofluorescin diacetate (DCFH-DA), Indo-1 and Fluo 3-AM, TCS-induced changes in nuclear morphology, reactive oxygen species (ROS) and intracellular calcium concentration ( [Ca2+](i)) during the apoptosis of choriocarcinoma cells (JAR) were simultaneously observed for the first time. The results indicated that TCS-induced increase in [Ca2+](i) and ROS formation were involved in apoptosis of JAR cells, and that TCS-induced ROS formation was related to TCS-evoked increase in, [Ca2+](i). Further studies with confocal. laser scanning microscopy revealed that TCS-evoked increase [Ca2+](i) was not the main factor responsible for TCS-induced ROS formation, and that TCS might induce the production of ROS through its interaction with membrane-bound receptor.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000171955800025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 1; 05; 717-721; 28
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/211392]
专题	生命科学学院
推荐引用方式 GB/T 7714	张春阳,贡宜萱,马辉,等. 双光子及共聚焦激光扫描显微术研究天花粉蛋白对人绒癌细胞的作用机制, Studying the effect of trichosanthin on choriocarcinoma cells with both two-photon and confocal laser scanning microscopy[J]. 生物化学与生物物理进展,2001.
APA	张春阳,贡宜萱,马辉,安成才,&陈瓞延.(2001).双光子及共聚焦激光扫描显微术研究天花粉蛋白对人绒癌细胞的作用机制.生物化学与生物物理进展.
MLA	张春阳,et al."双光子及共聚焦激光扫描显微术研究天花粉蛋白对人绒癌细胞的作用机制".生物化学与生物物理进展 (2001).