The Effects of Morin on Lipopolysaccharide-Induced Acute Lung Injury by   Suppressing the Lung NLRP3 Inflammasome

doi:10.1007/s10753-014-9930-1

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	The Effects of Morin on Lipopolysaccharide-Induced Acute Lung Injury by Suppressing the Lung NLRP3 Inflammasome
	Zhang Tianzhu ; Yang Shihai ; Du Juan
刊名	inflammation
	2014
关键词	morin NLRP3 inflammasome LPS-induced acute lung injury ENDOTOXEMIA HEMORRHAGE RESPONSES ALPHA
DOI	10.1007/s10753-014-9930-1
英文摘要	In previous study, the anti-inflammatory effect of morin had been found. In this study, we investigated anti-inflammatory effects of morin on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta, IL-18, and IL-6 were assayed by enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed by hematoxylin and eosin (HE) staining. The protein level of lung NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) inflammasome was measured by Western blotting. The data showed that treatment with the morin markedly attenuated inflammatory cell numbers in the BALF, decreased lung NLRP3 inflammasome protein level, and improved SOD activity and inhibited MPO activity. Histological studies demonstrated that morin substantially inhibited LPS-induced neutrophils in lung tissue compared with model group. The results indicated that the morin had a protective effect on LPS-induced ALI in mice.; Cell Biology; Immunology; SCI(E); 0; ARTICLE; zhangtz452@126.com; 6; 1976-1983; 37
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/207743]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang Tianzhu,Yang Shihai,Du Juan. The Effects of Morin on Lipopolysaccharide-Induced Acute Lung Injury by Suppressing the Lung NLRP3 Inflammasome[J]. inflammation,2014.
APA	Zhang Tianzhu,Yang Shihai,&Du Juan.(2014).The Effects of Morin on Lipopolysaccharide-Induced Acute Lung Injury by Suppressing the Lung NLRP3 Inflammasome.inflammation.
MLA	Zhang Tianzhu,et al."The Effects of Morin on Lipopolysaccharide-Induced Acute Lung Injury by Suppressing the Lung NLRP3 Inflammasome".inflammation (2014).