PPF1 inhibits programmed cell death in apical meristems of both G2 pea   transgenic Arabidopsis plants possibly by delaying cytosolic Ca2+   elevation

doi:10.1016/j.ceca.2003.07.003

CORC > 北京大学 > 生命科学学院

	PPF1 inhibits programmed cell death in apical meristems of both G2 pea transgenic Arabidopsis plants possibly by delaying cytosolic Ca2+ elevation
	Li, J ; Wang, DY ; Li, Q ; Xu, YJ ; Cui, KM ; Zhu, YX
刊名	cell calcium
	2004
关键词	apical meristem G2 pea PPF1 programmed cell death senescence calcium GATED ION-CHANNEL HYPERSENSITIVE RESPONSE SIGNAL-TRANSDUCTION SENESCENCE APOPTOSIS CALCIUM EXPRESSION MITOCHONDRIA ENDONUCLEASE MECHANISMS
DOI	10.1016/j.ceca.2003.07.003
英文摘要	PPF1 encodes a putative calcium ion carrier that affects the flowering time of transgenic Arabidopsis by modulating Ca2+ storage capacities in chloroplasts of a plant cell. In the current work, we found that differential expression of PPF1 might affect processes of programmed cell death (PCD) since DNA fragmentation was detected in senescencing apical buds of long day-grown G2 pea (Pisum sativum L.) plants, but was not in non-senescencing short day-grown counterparts at all growth stages. An animal inhibitor of caspase-activated DNase (ICAD) homologue was detected in short day-grown plant continuously throughout the whole experiment and only in early stages of long day-grown pre-floral G2 pea apical buds. DNA fragmentation was significantly inhibited in apical meristems of transgenic Arabidopsis that over-expressed the PPF1 gene when compared to that of either wild-type control or to PPF1(-) plants. The expression of ICAD-like protein decreased to undetectable level at 45 dpg in apical tissues of PPF1(-) Arabidopsis, which was much earlier than that found in PPF1(+) or wild-type controls. In epidermal cells of PPF1(-) plants, we recorded significantly earlier calcium transient prior to PCD. We suggest that the expression of PPF1, a chloroplast localized Ca2+ ion channel may inhibit programmed cell death in apical meristems of flowering plants by keeping a low cytoplasmic calcium content that might inhibit DNA fragmentation in plant cells. (C) 2003 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000188294400008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 21; ARTICLE; 1; 71-77; 35
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200049]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, J,Wang, DY,Li, Q,et al. PPF1 inhibits programmed cell death in apical meristems of both G2 pea transgenic Arabidopsis plants possibly by delaying cytosolic Ca2+ elevation[J]. cell calcium,2004.
APA	Li, J,Wang, DY,Li, Q,Xu, YJ,Cui, KM,&Zhu, YX.(2004).PPF1 inhibits programmed cell death in apical meristems of both G2 pea transgenic Arabidopsis plants possibly by delaying cytosolic Ca2+ elevation.cell calcium.
MLA	Li, J,et al."PPF1 inhibits programmed cell death in apical meristems of both G2 pea transgenic Arabidopsis plants possibly by delaying cytosolic Ca2+ elevation".cell calcium (2004).