The timing of endocytosis after activation of a G-protein-coupled   receptor in a sensory neuron

doi:10.1529/biophysj.105.069476

CORC > 北京大学 > 生命科学学院

	The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron
	Wang, LC ; Xiong, W ; Zheng, J ; Zhou, Y ; Zheng, H ; Zhang, C ; Zheng, LH ; Zhu, XL ; Xiong, ZQ ; Wang, LY ; Cheng, HP ; Zhou, Z
刊名	biophysical journal
	2006
关键词	ROOT GANGLION NEURONS ADRENAL CHROMAFFIN CELLS CLATHRIN-MEDIATED ENDOCYTOSIS FROG NEUROMUSCULAR JUNCTION TRANSMITTER RELEASE RAPID ENDOCYTOSIS NERVE-TERMINALS CENTRAL SYNAPSE CALCIUM INFLUX RAT ADIPOCYTES
DOI	10.1529/biophysj.105.069476
英文摘要	Endocytosis is a fundamental cellular event in membrane retrieval after exocytosis and in the regulation of receptor-mediated signal transduction. In contrast to the well-studied depolarization-induced membrane recycling, little is known about the kinetics of ligand-induced endocytosis of G-protein-coupled receptors in neurons. Here we investigated the kinetics of ligand-receptor binding-induced endocytosis in rat sensory neurons using a membrane capacitance assay. The time constant of ADP-induced endocytosis of P2Y-receptors was determined as 1.7 s. The ADP-induced endocytosis was blocked by antagonists against P2Y, phosphorylation, and clathrin. However, block of dynamin was without effect. The ADP-induced endocytosis was confirmed independently by a single vesicle image technique using a styryl FM2-10. Finally, the receptors were internalized in response to ADP, as determined by GFP-labeled P2Y. We conclude that ligand-receptor binding leads to rapid endocytosis in the cytoplasm of rat dorsal root ganglion neurons.; Biophysics; SCI(E); PubMed; 4; ARTICLE; 10; 3590-3598; 90
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198922]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, LC,Xiong, W,Zheng, J,et al. The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron[J]. biophysical journal,2006.
APA	Wang, LC.,Xiong, W.,Zheng, J.,Zhou, Y.,Zheng, H.,...&Zhou, Z.(2006).The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron.biophysical journal.
MLA	Wang, LC,et al."The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron".biophysical journal (2006).