Solution structure and functional characterization of Jingzhaotoxin-XI:   A novel gating modifier of both potassium and sodium channels

doi:10.1021/bi061457+

CORC > 北京大学 > 生命科学学院

	Solution structure and functional characterization of Jingzhaotoxin-XI: A novel gating modifier of both potassium and sodium channels
	Liao, Zhi ; Yuan, Chunhua ; Deng, Meichun ; Li, Jiang ; Chen, Jinjun ; Yang, Yuejun ; Hu, Weijun ; Liang, Songping
刊名	biochemistry us
	2006
关键词	DEPENDENT K+ CHANNELS MOLECULAR DETERMINANTS TARANTULA TOXINS SCORPION TOXIN VOLTAGE RECEPTOR ACTIVATION BINDING RAT INACTIVATION
DOI	10.1021/bi061457+
英文摘要	JZTX-XI is a peptide toxin isolated from the venom of the Chinese spider Chilobrachys jingzhao. It contains 34 residues including six cysteine residues with disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Using 3'- and 5'-RACE methods, the full-length cDNA was identified as encoding an 86-residue precursor of JZTX-XI. In the electrophysiological assay, JZTX-XI shows activity toward the Kv2.1 channel in a way similar to hanatoxin1 and SGTx1 that both the activation and the deactivation processes are affected, which is in accordance with the high sequence homology among them (over 60% identity). On the other hand, JZTX-XI also exhibits specific interaction against the Nav channels of rat cardiac myocytes with a significant reduction in the peak current and slowing of channel inactivation. The solution structure of native JZTX-XI was determined by H-1 NMR methods to identify the structural basis of these specific activities. Structural comparison of JZTX-XI with other gating modifier toxins shows that they all adopt a similar surface profile, a hydrophobic patch surrounded by charged residues such as Arg or Lys, which might be a common structural factor responsible for toxin-channel interaction. JZTX-XI might be an ideal tool to further investigate how spider toxins recognize various ion channels as their targets.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000242935600046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 24; ARTICLE; 51; 15591-15600; 45
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198544]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liao, Zhi,Yuan, Chunhua,Deng, Meichun,et al. Solution structure and functional characterization of Jingzhaotoxin-XI: A novel gating modifier of both potassium and sodium channels[J]. biochemistry us,2006.
APA	Liao, Zhi.,Yuan, Chunhua.,Deng, Meichun.,Li, Jiang.,Chen, Jinjun.,...&Liang, Songping.(2006).Solution structure and functional characterization of Jingzhaotoxin-XI: A novel gating modifier of both potassium and sodium channels.biochemistry us.
MLA	Liao, Zhi,et al."Solution structure and functional characterization of Jingzhaotoxin-XI: A novel gating modifier of both potassium and sodium channels".biochemistry us (2006).