Rice black-streaked dwarf virus outer capsid protein P10 has   self-interactions and forms oligomeric complexes in solution

doi:10.1016/j.virusres.2007.03.017

CORC > 北京大学 > 生命科学学院

	Rice black-streaked dwarf virus outer capsid protein P10 has self-interactions and forms oligomeric complexes in solution
	Liu, Huijun ; Wei, Chunhong ; Zhong, Yongwang ; Li, Yi
刊名	virus research
	2007
关键词	Rice black-streaked dwarf virus P10 outer capsid protein virion assembly protein self-interaction P10 oligomer MAJOR STRUCTURAL PROTEINS ATOMIC-STRUCTURE BACULOVIRUS EXPRESSION CRYSTAL-STRUCTURE ROTAVIRUS PARTICLES GENOME PHYTOREOVIRUS REVEALS BINDING
DOI	10.1016/j.virusres.2007.03.017
英文摘要	The P10 protein encoded by S10 ORF of Rice black-streaked dwarf virus (RBSDV) was thought to be the component of outer shell of viral particle. In the present study, P10 has an ability for self-interaction as shown by a GAL4 transcription activator-based yeast two-hybrid assay system and further confirmed by in vitro far-Western blot analysis. The domain responsible for P10-P10 self-interaction was mapped to the first 230 amino acids at the N-terminal region of the protein. The oligomerization property of PI 0 was further investigated using chemical cross-linking with purified recombinant P10 proteins expressed in a baculovirus expression system and glutaraldehyde. Intact P10 recombinants existed predominantly as trimers in solution in the absence of other viral proteins and displayed the oligomeric nature common to all known second-layer protein units of the Reoviridae. A truncated PI 0 mutant encoding the first 230 N-terminal amino acids lost its ability to form trimers even though dimeric, forms were detected during the cross-linking assay. Polyacrylamide gel electrophoresis under reducing or non-reducing conditions suggested that P10 subunits were oligomerized not through intermolecular disulfide bonds, but perhaps through some other type of association, such as hydrophobic or charge interactions. (C) 2007 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000247300900005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Virology; SCI(E); PubMed; 24; ARTICLE; 1; 34-42; 127
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198423]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Huijun,Wei, Chunhong,Zhong, Yongwang,et al. Rice black-streaked dwarf virus outer capsid protein P10 has self-interactions and forms oligomeric complexes in solution[J]. virus research,2007.
APA	Liu, Huijun,Wei, Chunhong,Zhong, Yongwang,&Li, Yi.(2007).Rice black-streaked dwarf virus outer capsid protein P10 has self-interactions and forms oligomeric complexes in solution.virus research.
MLA	Liu, Huijun,et al."Rice black-streaked dwarf virus outer capsid protein P10 has self-interactions and forms oligomeric complexes in solution".virus research (2007).