Rice black-streaked dwarf virus minor core protein P8 is a nuclear   dimeric protein and represses transcription in tobacco protoplasts

doi:10.1016/j.febslet.2007.04.071

CORC > 北京大学 > 生命科学学院

	Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts
	Liu, Huijun ; Wei, Chunhong ; Zhong, Yongwang ; Li, Yi
刊名	欧洲生化学会联合会快报
	2007
关键词	RBSDV P8 minor core protein nuclear targeting transcriptional repression dimer GENE-EXPRESSION NONSTRUCTURAL PROTEIN FUNCTIONAL-ANALYSIS COMPLETE GENOME HIGHER-PLANTS DOMAIN SEQUENCE IDENTIFICATION LOCALIZATION SUPPRESSION
DOI	10.1016/j.febslet.2007.04.071
英文摘要	Virus-encoding nuclear transcriptional regulators play important roles in the viral life cycle. Most of these proteins exhibit intrinsic transcriptional activation or repression activity, and are involved in the regulation of the expression of virus genome itself or important cellular genes to facilitate viral replication and inhibit antiviral responses. Here, we report that the minor core protein P8 of Rice black-streaked dwarf virus, a dsRNA virus infecting host plants and insects, is targeted to the nucleus of insect and plant cells via its N-terminal 1-40 amino acids and possesses potent active transcriptional repression activity in Bright Yellow-2 tobacco suspension cells. Moreover, P8, like many transcriptional regulatory proteins, is capable of forming homo-dimers within insect cells and in vitro. All these data suggest that P8 is likely to enter the nucleus of host cell and play an important role as a negative transcriptional regulator of host gene expression during the process of virus-host interaction. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000247087600025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); PubMed; 17; ARTICLE; 13; 2534-2540; 581
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198362]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Huijun,Wei, Chunhong,Zhong, Yongwang,et al. Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts[J]. 欧洲生化学会联合会快报,2007.
APA	Liu, Huijun,Wei, Chunhong,Zhong, Yongwang,&Li, Yi.(2007).Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts.欧洲生化学会联合会快报.
MLA	Liu, Huijun,et al."Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts".欧洲生化学会联合会快报 (2007).