Inhibition of protein deacetylation by trichostatin A impairs   microtubule-kinetochore attachment

doi:10.1007/s00018-008-8237-5

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	Inhibition of protein deacetylation by trichostatin A impairs microtubule-kinetochore attachment
	Ma, Y. ; Cai, S. ; Lu, Q. ; Lu, X. ; Jiang, Q. ; Zhou, J. ; Zhang, C.
刊名	cellular and molecular life sciences
	2008
关键词	trichostatin A deacetylase inhibitor kinetochore chromosome passenger complex mitotic centromere-associated kinesin CHROMOSOMAL PASSENGER COMPLEX AURORA-B MITOTIC SPINDLE CELL-CYCLE ESSENTIAL ROLES MOTOR PROTEINS CENP-H CENTROMERE SEGREGATION DYNAMICS
DOI	10.1007/s00018-008-8237-5
英文摘要	Inhibition of protein deacetylation arrests cells in mitosis, but the mechanism is unknown. To understand why inhibiting protein deacetylation causes cell cycle arrest, we treated HeLa cells beyond G1/S transition with trichostatin A (TSA), a potent protein deacetylase inhibitor, and found that the cells arrested at prometaphase with ectopic spindles and unaligned chromosomes. The hyper-acetylated cells encountered a serious microtubule (MT)-kinetochore attachment problem, although the kinetochores are intact at ultrastructural level. By immunofluorescence staining of kinetochore proteins, we found that the pericentromeric H3K9Me3-HP1 pathway was disrupted and that the CENP-A-dependent outer plate protein dynamics of kinetochores was greatly diminished by the drug treatment. The treatment also caused the loss of chromosome passenger complex (CPC), the proposed error checking system, from centromere and impaired the microtubule dynamics of the cells. Overall, we propose that deacetylation inhibition impairs MT-kinetochore attachment through disrupting the centromere function and altering the kinetochore composition and MT dynamics.; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 12; ARTICLE; 19; 3100-3109; 65
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/197567]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ma, Y.,Cai, S.,Lu, Q.,et al. Inhibition of protein deacetylation by trichostatin A impairs microtubule-kinetochore attachment[J]. cellular and molecular life sciences,2008.
APA	Ma, Y..,Cai, S..,Lu, Q..,Lu, X..,Jiang, Q..,...&Zhang, C..(2008).Inhibition of protein deacetylation by trichostatin A impairs microtubule-kinetochore attachment.cellular and molecular life sciences.
MLA	Ma, Y.,et al."Inhibition of protein deacetylation by trichostatin A impairs microtubule-kinetochore attachment".cellular and molecular life sciences (2008).