Novel AroA from Pseudomonas putida Confers Tobacco Plant with High   Tolerance to Glyphosate

doi:10.1371/journal.pone.0019732

CORC > 北京大学 > 生命科学学院

	Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate
	Yan, Hai-Qin ; Chang, Su-Hua ; Tian, Zhe-Xian ; Zhang, Le ; Sun, Yi-Cheng ; Li, Yan ; Wang, Jing ; Wang, Yi-Ping
刊名	plos one
	2011
关键词	RAPESEED BRASSICA-NAPUS ESCHERICHIA-COLI K12 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE HERBICIDE GLYPHOSATE 3-PHOSPHATE SYNTHASE TRANSGENIC PLANTS CODON USAGE DNA TRANSFORMATION SUBSTITUTION
DOI	10.1371/journal.pone.0019732
英文摘要	Glyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, also designated as AroA), a key enzyme in the aromatic amino acid biosynthesis pathway in microorganisms and plants. Previously, we reported that a novel AroA (PpAroA1) from Pseudomonas putida had high tolerance to glyphosate, with little homology to class I or class II glyphosate-tolerant AroA. In this study, the coding sequence of PpAroA1 was optimized for tobacco. For maturation of the enzyme in chloroplast, a chloroplast transit peptide coding sequence was fused in frame with the optimized aroA gene (PparoA1(optimized)) at the 59 end. The PparoA1(optimized) gene was introduced into the tobacco (Nicotiana tabacum L. cv. W38) genome via Agrobacterium-mediated transformation. The transformed explants were first screened in shoot induction medium containing kanamycin. Then glyphosate tolerance was assayed in putative transgenic plants and its T-1 progeny. Our results show that the PpAroA1 from Pseudomonas putida can efficiently confer tobacco plants with high glyphosate tolerance. Transgenic tobacco overexpressing the PparoA1(optimized) gene exhibit high tolerance to glyphosate, which suggest that the novel PpAroA1 is a new and good candidate applied in transgenic crops with glyphosate tolerance in future.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000290720200029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 3; ARTICLE; 5; e19732; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/193699]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yan, Hai-Qin,Chang, Su-Hua,Tian, Zhe-Xian,et al. Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate[J]. plos one,2011.
APA	Yan, Hai-Qin.,Chang, Su-Hua.,Tian, Zhe-Xian.,Zhang, Le.,Sun, Yi-Cheng.,...&Wang, Yi-Ping.(2011).Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate.plos one.
MLA	Yan, Hai-Qin,et al."Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate".plos one (2011).