The Intracellular Transport and Secretion of Calumenin-1/2 in Living   Cells

doi:10.1371/journal.pone.0035344

CORC > 北京大学 > 生命科学学院

	The Intracellular Transport and Secretion of Calumenin-1/2 in Living Cells
	Wang, Qiao ; Feng, Hui ; Zheng, Pengli ; Shen, Birong ; Chen, Liang ; Liu, Lin ; Liu, Xiao ; Hao, Qingsong ; Wang, Shunchang ; Chen, Jianguo ; Teng, Junlin
刊名	plos one
	2012
关键词	ENDOPLASMIC-RETICULUM CYTOPLASMIC DYNEIN SARCOPLASMIC-RETICULUM CA2+-BINDING PROTEIN GOLGI TRANSPORT COATED VESICLES BULK FLOW KINESIN ER MEMBRANE
DOI	10.1371/journal.pone.0035344
英文摘要	Calumenin isoforms 1 and 2 (calu-1/2), encoded by the CALU gene, belong to the CREC protein family. Calu-1/2 proteins are secreted into the extracellular space, but the secretory process and regulatory mechanism are largely unknown. Here, using a time-lapse imaging system, we visualized the intracellular transport and secretory process of calu-1/2-EGFP after their translocation into the ER lumen. Interestingly, we observed that an abundance of calu-1/2-EGFP accumulated in cellular processes before being released into the extracellular space, while only part of calu-1/2-EGFP proteins were secreted directly after attaching to the cell periphery. Moreover, we found the secretion of calu-1/2-EGFP required microtubule integrity, and that calu-1/2-EGFP-containing vesicles were transported by the motor proteins Kif5b and cytoplasmic dynein. Finally, we determined the export signal of calu-1/2-EGFP (amino acid positions 20-46) and provided evidence that the asparagine at site 131 was indispensable for calu-1/2-EGFP stabilization. Taken together, we provide a detailed picture of the intracellular transport of calu-1/2-EGFP, which facilitates our understanding of the secretory mechanism of calu-1/2.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305341600137&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 7; ARTICLE; 4; e35344; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/192034]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Qiao,Feng, Hui,Zheng, Pengli,et al. The Intracellular Transport and Secretion of Calumenin-1/2 in Living Cells[J]. plos one,2012.
APA	Wang, Qiao.,Feng, Hui.,Zheng, Pengli.,Shen, Birong.,Chen, Liang.,...&Teng, Junlin.(2012).The Intracellular Transport and Secretion of Calumenin-1/2 in Living Cells.plos one.
MLA	Wang, Qiao,et al."The Intracellular Transport and Secretion of Calumenin-1/2 in Living Cells".plos one (2012).