Influences of calcitonin gene-related peptide on mu opioid receptors in   nucleus accumbens neurons of rats

doi:10.1016/j.npep.2012.10.008

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	Influences of calcitonin gene-related peptide on mu opioid receptors in nucleus accumbens neurons of rats
	Yan, Hailiang ; Yu, Long-Chuan
刊名	neuropeptides
	2013
关键词	Calcitonin gene-related peptide Calcitonin receptor-like receptor Mu opioid receptor Nucleus accumbens Receptor activity-modifying protein Morphine tolerance ANTINOCICEPTIVE ACTIVITY MORPHINE ANALGESIA DORSAL-HORN DESENSITIZATION INHIBITION TOLERANCE CGRP MICE PHOSPHORYLATION LOCALIZATION
DOI	10.1016/j.npep.2012.10.008
英文摘要	The Mu opioid receptor (MOR) has been shown to participate in the analgesic effect of the calcitonin gene-related peptide (CGRP) in the nucleus accumbens (NAc) of adult rats. However, it is not clear whether and how CGRP regulates the MOR at the molecular levels. In the present study, it is found that the level of MORs on the cell membrane of NAc neurons was increased twice more than the control level following CGRP treatment (1 mu M, 30 min), which is a phenomenon that was blocked by the peptidergic antagonist CGRP(8-37). No direct physical interaction was observed between MORs and CGRP receptors, and neither brefeldin A nor dynosore preincubation affected such effects of CGRP. However, addition of 20 mu M monensin 1 h before CGRP treatment significantly blocked the action of CGRP on surface MORs. In living animals, microinjection of CGRP (1 nmol in 1 mu l) into the NAc partially restored morphine antinociception in morphine-tolerant rats, and the effect of CGRP on surface MORs extended beyond normal NAc neurons to chronic morphine-treated NAc neurons. To conclude, these results demonstrate that CGRP can act on MORs and increase the number of surface MORs in NAc neurons, partially explaining the involvement of opioid receptors in CGRP-induced antinociception in the rat NAc. (C) 2012 Elsevier Ltd. All rights reserved.; Endocrinology & Metabolism; Neurosciences; SCI(E); PubMed; 3; ARTICLE; 2; 125-131; 47
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190760]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yan, Hailiang,Yu, Long-Chuan. Influences of calcitonin gene-related peptide on mu opioid receptors in nucleus accumbens neurons of rats[J]. neuropeptides,2013.
APA	Yan, Hailiang,&Yu, Long-Chuan.(2013).Influences of calcitonin gene-related peptide on mu opioid receptors in nucleus accumbens neurons of rats.neuropeptides.
MLA	Yan, Hailiang,et al."Influences of calcitonin gene-related peptide on mu opioid receptors in nucleus accumbens neurons of rats".neuropeptides (2013).