Crystallization and X-ray crystallographic analysis of the cap-binding   domain of influenza A virus H1N1 polymerase subunit PB2

doi:10.1107/S1744309113002388

CORC > 北京大学 > 生命科学学院

	Crystallization and X-ray crystallographic analysis of the cap-binding domain of influenza A virus H1N1 polymerase subunit PB2
	Liu, Yong ; Meng, Geng ; Luo, Ming ; Zheng, Xiaofeng
刊名	acta crystallographica section f structural biology and crystallization communications
	2013
关键词	PB2 cap-binding protein PROTEIN EIF4E ENDONUCLEASE SITE
DOI	10.1107/S1744309113002388
英文摘要	PB2 is one of the subunits of the influenza virus heterotrimeric polymerase. By its cap-binding domain (PB2cap), PB2 captures the 5 cap of the host pre-mRNA to generate a capped 5 oligonucleotide primer for virus transcription. The crystal structure of influenza A virus H3N2 PB2cap with bound cap analogue m7GTP has been reported previously. To show the substrate-free structural details of PB2cap and clarify whether obvious conformational changes exist between the substrate-free and substrate-bound cap-binding domain, we have successfully obtained the crystal of substrate-free H1N1 PB2cap. The crystal of H1N1 PB2cap diffracted to a high resolution of 1.32 angstrom. The crystal symmetry belongs to space group P1 with unit-cell parameters a = 29.49, b = 37.04, c = 38.33 angstrom, = 71.10, = 69.84, = 75.85 degrees. There is one molecule in the asymmetric unit.; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 2; ARTICLE; Pt 3; 280-283; 69
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190364]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Yong,Meng, Geng,Luo, Ming,et al. Crystallization and X-ray crystallographic analysis of the cap-binding domain of influenza A virus H1N1 polymerase subunit PB2[J]. acta crystallographica section f structural biology and crystallization communications,2013.
APA	Liu, Yong,Meng, Geng,Luo, Ming,&Zheng, Xiaofeng.(2013).Crystallization and X-ray crystallographic analysis of the cap-binding domain of influenza A virus H1N1 polymerase subunit PB2.acta crystallographica section f structural biology and crystallization communications.
MLA	Liu, Yong,et al."Crystallization and X-ray crystallographic analysis of the cap-binding domain of influenza A virus H1N1 polymerase subunit PB2".acta crystallographica section f structural biology and crystallization communications (2013).