The Chromatin Remodeling Factor CSB Recruits Histone Acetyltransferase   PCAF to rRNA Gene Promoters in Active State for Transcription Initiation

doi:10.1371/journal.pone.0062668

CORC > 北京大学 > 生命科学学院

	The Chromatin Remodeling Factor CSB Recruits Histone Acetyltransferase PCAF to rRNA Gene Promoters in Active State for Transcription Initiation
	Shen, Meili ; Zhou, Tingting ; Xie, Wenbing ; Ling, Te ; Zhu, Qiaoyun ; Zong, Le ; Lyu, Guoliang ; Gao, Qianqian ; Zhang, Feixiong ; Tao, Wei
刊名	plos one
	2013
关键词	POLYMERASE-I TRANSCRIPTION GROUP-B PROTEIN COCKAYNE-SYNDROME COUPLING FACTOR DNA-DAMAGE REPAIR COMPLEX ACETYLATION BROMODOMAIN BINDING
DOI	10.1371/journal.pone.0062668
英文摘要	The promoters of poised rRNA genes (rDNA) are marked by both euchromatic and heterochromatic histone modifications and are associated with two transcription factors, UBF and SL1 that nucleate transcription complex formation. Active rRNA genes contain only euchromatic histone modifications and are loaded with all components of transcriptional initiation complex including RNA polymerase I. Coupled with histone acetylation and RNA polymerase I targeting, poised promoters can be converted to active ones by ATP-dependent chromatin remodeling factor CSB for initiation of rDNA transcription. However, it is not clear how dynamic histone modifications induce the assembly of polymerase I transcription initiation complex to active promoters during such conversion. Here we show that a complex consisting of CSB, RNA polymerase I and histone acetyltransferase PCAF is present at the rDNA promoters in active state. CSB is required for the association of PCAF with rDNA, which induces acetylation of histone H4 and histone H3K9. Overexpression of CSB promotes the association of PCAF with rDNA. Knockdown of PCAF leads to decreased levels of H4ac and H3K9ac at rDNA promoters, prevents the association of RNA polymerase I and inhibits pre-rRNA synthesis. The results demonstrate that CSB recruits PCAF to rDNA, which allows histone acetylation that is required for the assembly of polymerase I transcription initiation complex during the transition from poised to active state of rRNA genes, suggesting that CSB and PCAF play cooperative roles to establish the active state of rRNA genes by histone acetylation.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000319654700053&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 3; ARTICLE; 5; e62668; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190176]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shen, Meili,Zhou, Tingting,Xie, Wenbing,et al. The Chromatin Remodeling Factor CSB Recruits Histone Acetyltransferase PCAF to rRNA Gene Promoters in Active State for Transcription Initiation[J]. plos one,2013.
APA	Shen, Meili.,Zhou, Tingting.,Xie, Wenbing.,Ling, Te.,Zhu, Qiaoyun.,...&Tao, Wei.(2013).The Chromatin Remodeling Factor CSB Recruits Histone Acetyltransferase PCAF to rRNA Gene Promoters in Active State for Transcription Initiation.plos one.
MLA	Shen, Meili,et al."The Chromatin Remodeling Factor CSB Recruits Histone Acetyltransferase PCAF to rRNA Gene Promoters in Active State for Transcription Initiation".plos one (2013).