Reproducible copy number variation patterns among single circulating   tumor cells of lung cancer patients

doi:10.1073/pnas.1320659110

CORC > 北京大学 > 生命科学学院

	Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients
	Ni, Xiaohui ; Zhuo, Minglei ; Su, Zhe ; Duan, Jianchun ; Gao, Yan ; Wang, Zhijie ; Zong, Chenghang ; Bai, Hua ; Chapman, Alec R. ; Zhao, Jun ; Xu, Liya ; An, Tongtong ; Ma, Qi ; Wang, Yuyan ; Wu, Meina ; Sun, Yu ; Wang, Shuhang ; Li, Zhenxiang ; Yang, Xiaodan ; Yong, Jun ; Su, Xiao-Dong ; Lu, Youyong ; Bai, Fan ; Xie, X. Sunney ; Wang, Jie
刊名	proceedings of the national academy of sciences of the united states of america
	2013
关键词	cancer diagnostics personalized therapy METASTATIC BREAST-CANCER PLASMA DNA HUMAN GENOME MUTATIONS NUCLEOTIDE HETEROGENEITY PROGRESSION THERAPY GENE
DOI	10.1073/pnas.1320659110
英文摘要	Circulating tumor cells (CTCs) enter peripheral blood from primary tumors and seed metastases. The genome sequencing of CTCs could offer noninvasive prognosis or even diagnosis, but has been hampered by low single-cell genome coverage of scarce CTCs. Here, we report the use of the recently developed multiple annealing and looping-based amplification cycles for whole-genome amplification of single CTCs from lung cancer patients. We observed characteristic cancer-associated single-nucleotide variations and insertions/deletions in exomes of CTCs. These mutations provided information needed for individualized therapy, such as drug resistance and phenotypic transition, but were heterogeneous from cell to cell. In contrast, every CTC from an individual patient, regardless of the cancer subtypes, exhibited reproducible copy number variation (CNV) patterns, similar to those of the metastatic tumor of the same patient. Interestingly, different patients with the same lung cancer adenocarcinoma (ADC) shared similar CNV patterns in their CTCs. Even more interestingly, patients of small-cell lung cancer have CNV patterns distinctly different from those of ADC patients. Our finding suggests that CNVs at certain genomic loci are selected for the metastasis of cancer. The reproducibility of cancer-specific CNVs offers potential for CTC-based cancer diagnostics.; Multidisciplinary Sciences; SCI(E); PubMed; 45; ARTICLE; 52; 21083-21088; 110
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189741]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ni, Xiaohui,Zhuo, Minglei,Su, Zhe,et al. Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients[J]. proceedings of the national academy of sciences of the united states of america,2013.
APA	Ni, Xiaohui.,Zhuo, Minglei.,Su, Zhe.,Duan, Jianchun.,Gao, Yan.,...&Wang, Jie.(2013).Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients.proceedings of the national academy of sciences of the united states of america.
MLA	Ni, Xiaohui,et al."Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients".proceedings of the national academy of sciences of the united states of america (2013).