A mammary repopulating cell population characterized in mammary anlagen   reveals essential mammary stroma for morphogenesis

doi:10.1016/j.yexcr.2014.06.008

CORC > 北京大学 > 生命科学学院

	A mammary repopulating cell population characterized in mammary anlagen reveals essential mammary stroma for morphogenesis
	Song, Jiazhe ; Xue, Kai ; She, Ji ; Ding, Fangrong ; Li, Song ; Shangguan, Rulan ; Dai, Yunping ; Du, Liying ; Li, Ning
刊名	experimental cell research
	2014
关键词	Mammary gland morphogenesis Mammary repopulating units (MRU) Mammary anlagen stage Mammary stem/progenitor cell Mammary stroma HEMATOPOIETIC STEM-CELLS GLAND DEVELOPMENT BREAST-CANCER PROLACTIN RECEPTOR TISSUE INTERACTION MOUSE EMBRYO ACTIVATION EXPRESSION EMBRYOGENESIS TESTOSTERONE
DOI	10.1016/j.yexcr.2014.06.008
英文摘要	The cells with mammary repopulating capability can achieve mammary gland morphogenesis in a suitable cellular microenvironment. Using cell surface markers of CD24, CD29 and CD49f, mouse mammary repopulating unit (MRU) has been identified in adult mammary epithelium and late embryonic mammary bud epithelium. However, embryonic MRU remains to be fully characterized at earlier mammary anlagen stage. Here we isolated discrete populations of E14.5 mouse mammary anlagen cells. Only Lin(-)CD24(med)CD29(+) cell population was predicted as E14.5 MRU by examining their capacities of forming mammosphere and repopulating cleared mammary fat pad in vivo. However, when we characterized gene expressions of this E14.5 cell population by comparing with adult mouse MRU (Lin(-)CD24(+)CD29(hi)), the gene profiling of these two cell populations exhibited great differences. Real-time PCR and immunostaining assays uncovered that E14.5 Lin(-)CD24(med)CD29(+) cell population was a heterogeneous stroma-enriched cell population. Then, limiting dilutions and single-cell assays also confirmed that E14.5 Lin(-)CD24(med)CD29(+) cell population possessed low proportion of stem cells. In summary, heterogeneous Lin(-)CD24(med)CD29(+) cell population exhibited mammary repopulating ability in E14.5 mammary anlagen, implying that only suitable mammary stroma could enable mammary gland morphogenesis, which relied on the interaction between rare stem cells and microenvironment. (C) 2014 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000341341700012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Oncology; Cell Biology; SCI(E); PubMed; 0; ARTICLE; ninglcau@cau.edu.cn; 1; 123-134; 327
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189358]
专题	生命科学学院
推荐引用方式 GB/T 7714	Song, Jiazhe,Xue, Kai,She, Ji,et al. A mammary repopulating cell population characterized in mammary anlagen reveals essential mammary stroma for morphogenesis[J]. experimental cell research,2014.
APA	Song, Jiazhe.,Xue, Kai.,She, Ji.,Ding, Fangrong.,Li, Song.,...&Li, Ning.(2014).A mammary repopulating cell population characterized in mammary anlagen reveals essential mammary stroma for morphogenesis.experimental cell research.
MLA	Song, Jiazhe,et al."A mammary repopulating cell population characterized in mammary anlagen reveals essential mammary stroma for morphogenesis".experimental cell research (2014).