Potential protection of 2,3,5,4   &apos;-tetrahydroxystilbene-2-O-beta-D-glucoside against   staurosporine-induced toxicity on cultured rat hippocampus neurons

doi:10.1016/j.neulet.2014.05.045

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	Potential protection of 2,3,5,4 '-tetrahydroxystilbene-2-O-beta-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons
	Yang, Xiao-Ping ; Liu, Tao-Yan ; Qin, Xiao-Yan ; Yu, Long-Chuan
刊名	神经科学快报
	2014
关键词	THSG STS Hippocampal neurons p-Akt Bcl(2) pro-Csp3 PREFRONTAL CORTICAL-NEURONS BETA-INDUCED TOXICITY TETRAHYDROXYSTILBENE GLUCOSIDE IMPROVES POLYGONUM-MULTIFLORUM ALZHEIMERS-DISEASE MOUSE MODEL CELLS MICE APOPTOSIS MEMORY
DOI	10.1016/j.neulet.2014.05.045
英文摘要	The present study explored the effect of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) on the staurosporine (STS)-induced toxicity in cultured rat hippocampal neurons. The results showed that administration of 200 mu M of THSG significantly protected against 0.3 mu M of STS-induced apoptosis in cultured rat hippocampal neurons tested by methyl thiazolyl tetrazolium (MTT) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays. Furthermore, when the Akt signaling pathway was blocked by LY294002, an inhibitor of Phosphatidyl Inositol 3-kinase (PI3K), the protective effects of THSG against STS-induced neurotoxicity were abrogated. We further examined the involvement of PI3K/Akt signaling pathway in THSG protection against STS-induced cytotoxicity on cultured neurons and found that administration of THSG significantly inhibited the STS-induced decreases in the content of phosphoiylated AKt (p-Akt). Moreover, we found that THSG rescued the down-regulation of B cell lymphoma/lewkmia-2 (Bcl(2)) and pro-caspase-3 (pro-Csp3) caused by STS in the neurons. These results indicate that THSG protect the cultured rat hippocampal neurons against STS-induced cytotoxicity and the PI3K/Akt signaling and mitochondrial apoptotic pathways are involved in the THSG-induced protective effects. (C) 2014 Elsevier Ireland Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000340853900015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Neurosciences; SCI(E); PubMed; 3; ARTICLE; zhongsijia01@163.com; yulc@pku.edu.cn; 79-83; 576
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188999]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, Xiao-Ping,Liu, Tao-Yan,Qin, Xiao-Yan,et al. Potential protection of 2,3,5,4 '-tetrahydroxystilbene-2-O-beta-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons[J]. 神经科学快报,2014.
APA	Yang, Xiao-Ping,Liu, Tao-Yan,Qin, Xiao-Yan,&Yu, Long-Chuan.(2014).Potential protection of 2,3,5,4 '-tetrahydroxystilbene-2-O-beta-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons.神经科学快报.
MLA	Yang, Xiao-Ping,et al."Potential protection of 2,3,5,4 '-tetrahydroxystilbene-2-O-beta-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons".神经科学快报 (2014).