Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation

doi:10.1016/j.stem.2015.03.007

CORC > 北京大学 > 生命科学学院

	Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation
	Yin Yafei ; Yan Pixi ; Lu Jinlong ; Song Guang ; Zhu Yangyang ; Li Zhaohui ; Zhao Yi ; Shen Bin ; Huang Xingxu ; Zhu Heng ; Orkin Stuart H ; Shen Xiaohua
刊名	cell stem cell
	2015
DOI	10.1016/j.stem.2015.03.007
英文摘要	Long noncoding RNAs (lncRNAs) have been implicated in controlling various aspects of embryonic stem cell (ESC) biology, although the functions of specific lncRNAs, and the molecular mechanisms through which they act, remain unclear. Here, we demonstrate discrete and opposing roles for the lncRNA transcript Haunt and its genomic locus in regulating the HOXA gene cluster during ESC differentiation. Reducing or enhancing Haunt expression, with minimal disruption of the Haunt locus, led to upregulation or downregulation of HOXA genes, respectively. In contrast, increasingly large genomic deletions within the Haunt locus attenuated HOXA activation. The Haunt DNA locus contains potential enhancers of HOXA activation, whereas Haunt RNA acts to prevent aberrant HOXA expression. This work reveals a multifaceted model of lncRNA-mediated transcriptional regulation of the HOXA cluster, with distinct roles for a lncRNA transcript and its genomic locus, while illustrating the power of rapid CRISPR/Cas9-based genome editing for assigning lncRNA functions.Copyright ? 2015 Elsevier Inc. All rights reserved.; SCI(E); PubMed; 0; ARTICLE; xshen@tsinghua.edu.cn; 5; 504-16; 16
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188050]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yin Yafei,Yan Pixi,Lu Jinlong,et al. Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation[J]. cell stem cell,2015.
APA	Yin Yafei.,Yan Pixi.,Lu Jinlong.,Song Guang.,Zhu Yangyang.,...&Shen Xiaohua.(2015).Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation.cell stem cell.
MLA	Yin Yafei,et al."Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation".cell stem cell (2015).