Quantitative proteomic analysis of human osteoblast-like MG-63 cells in   response to bioinert implant material titanium and polyetheretherketone

doi:10.1016/j.jprot.2012.03.033

CORC > 北京大学 > 生命科学学院

	Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone
	Zhao, Minzhi ; An, Mingrui ; Wang, Qingsong ; Liu, Xiaochen ; Lai, Wenjia ; Zhao, Xuyang ; Wei, Shicheng ; Ji, Jianguo
刊名	journal of proteomics
	2012
关键词	Biomaterial Biocompatibility SILAC Polyetheretherketone (PEEK) Titanium Proteomics MICROARRAY DATA PROTEIN BIOMATERIAL GENE
DOI	10.1016/j.jprot.2012.03.033
英文摘要	Commercially pure titanium (cpTi) and polyetheretherketone (PEEK) are widely used surface-modified implant materials in orthopedics and dental therapeutics. However, there still has not been comprehensive biocompatibility evaluation of them at molecular level. By employing stable isotope labeling with amino acids in cell culture (SILAC), we profiled the dynamic protein expression changes in human osteoblast-like MG-63 cells cultured on cpTi and PEEK, respectively. About 2000 proteins were quantified and 400 proteins showed substantial alterations in expression levels upon each material treatment Notably, the extent of alterations diminished as the contact prolonged, which suggested adaptive response to the bioinert materials. Similar patterns of expression changes were observed for both cpTi and PEEK. The representative pathways reflected the regulation of biosynthesis, metabolism and cell adhesion in the adaptive process. In addition, PEEK showed stronger inhibition on mRNA processing, which explained the lower proliferation rate of the cells cultured on PEEK. Our results indicated that the widely used bioinert materials cpTi and PEEK could individually induce a cooperative response involving a wide panel of proteins and pathways. This study has established a basis for better understanding the biocompatibility of surface-modified implant biomaterials at molecular level. (c) 2012 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000307086500014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; SCI(E); PubMed; 13; ARTICLE; 12; 3560-3573; 75
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/164090]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhao, Minzhi,An, Mingrui,Wang, Qingsong,et al. Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone[J]. journal of proteomics,2012.
APA	Zhao, Minzhi.,An, Mingrui.,Wang, Qingsong.,Liu, Xiaochen.,Lai, Wenjia.,...&Ji, Jianguo.(2012).Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone.journal of proteomics.
MLA	Zhao, Minzhi,et al."Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone".journal of proteomics (2012).