Structural Mechanism of Substrate RNA Recruitment in H/ACA RNA-Guided   Pseudouridine Synthase

doi:10.1016/j.molcel.2009.05.005

CORC > 北京大学 > 生命科学学院

	Structural Mechanism of Substrate RNA Recruitment in H/ACA RNA-Guided Pseudouridine Synthase
	Duan, Jingqi ; Li, Ling ; Lu, Jing ; Wang, Wei ; Ye, Keqiong
刊名	分子细胞
	2009
关键词	SMALL NUCLEOLAR RNAS CRYSTAL-STRUCTURE RIBOSOMAL-RNA SACCHAROMYCES-CEREVISIAE BINDING MOTIF COMPLEX SNORNA PROTEINS RECOGNITION ASSOCIATION
DOI	10.1016/j.molcel.2009.05.005
英文摘要	H/ACA RNAs form ribonucleoprotein complex (RNP) with proteins Cbf5, Nop10, L7Ae, and Gar1 and guide site-specific conversion of uridine into pseudouridine in cellular RNAs. The crystal structures of H/ACA RNP with substrate bound at the active site cleft reveal that the substrate is recruited through sequence-specific pairing with guide RNA and essential protein contacts. Substrate binding leads to a reorganization of a preset pseudouridylation pocket and an adaptive movement of the PUA domain and the lower stem of the H/ACA RNA. Moreover, a thumb loop flips from the Gar1-bound state in the substrate-free RNP structure to tightly associate with the substrate. Mutagenesis and enzyme kinetics analysis suggest a critical role of Gar1 and the thumb in substrate turnover, particularly in product release. Comparison with tRNA Psi 55 synthase TruB reveals the structural conservation and adaptation between an RNA-guided and stand-alone pseudouridine synthase and provides insight into the guide-independent activity of Cbf5.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000266617600008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 48; ARTICLE; 4; 427-439; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162577]
专题	生命科学学院
推荐引用方式 GB/T 7714	Duan, Jingqi,Li, Ling,Lu, Jing,et al. Structural Mechanism of Substrate RNA Recruitment in H/ACA RNA-Guided Pseudouridine Synthase[J]. 分子细胞,2009.
APA	Duan, Jingqi,Li, Ling,Lu, Jing,Wang, Wei,&Ye, Keqiong.(2009).Structural Mechanism of Substrate RNA Recruitment in H/ACA RNA-Guided Pseudouridine Synthase.分子细胞.
MLA	Duan, Jingqi,et al."Structural Mechanism of Substrate RNA Recruitment in H/ACA RNA-Guided Pseudouridine Synthase".分子细胞 (2009).