TALEN construction via &quot;Unit Assembly&quot; method and targeted genome   modifications in zebrafish

doi:10.1016/j.ymeth.2014.02.010

CORC > 北京大学 > 生命科学学院

	TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish
	Huang, Peng ; Xiao, An ; Tong, Xiangjun ; Zu, Yao ; Wang, Zhanxiang ; Zhang, Bo
刊名	methods
	2014
关键词	TALEN Zebrafish Gene targeting Homologous recombination Chromosomal deletion Genome manipulation CHROMOSOMAL DELETIONS DNA EFFICIENT DESIGN TRANSCRIPTION INVERSIONS PREDICTION NUCLEASES EFFECTORS SEQUENCE
DOI	10.1016/j.ymeth.2014.02.010
英文摘要	Transcription activator-like effector nucleases (TALENs) are engineered endonucleases composed of a customized transcription activator-like effector (TALE) DNA-binding domain and a Fold DNA cleavage domain. TALENs induce DNA double-strand breaks (DSBs) at their target sites on the chromosome and have been successfully used for genome engineering in many species and cultured cells. Zebrafish is a very popular model organism in both basic and clinical research. Here, we describe the details of construction of customized TALENs using the "Unit Assembly" (UA) method, as well as three applications of zebrafish genome manipulations using TALENs: gene knock-out, large chromosome deletion, and gene knock-in by homologous recombination. (C) 2014 Elsevier Inc. All rights reserved.; Biochemical Research Methods; Biochemistry & Molecular Biology; SCI(E); PubMed; 4; ARTICLE; bzhang@pku.edu.cn; 1; 67-75; 69
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162446]
专题	生命科学学院
推荐引用方式 GB/T 7714	Huang, Peng,Xiao, An,Tong, Xiangjun,et al. TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish[J]. methods,2014.
APA	Huang, Peng,Xiao, An,Tong, Xiangjun,Zu, Yao,Wang, Zhanxiang,&Zhang, Bo.(2014).TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish.methods.
MLA	Huang, Peng,et al."TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish".methods (2014).