低分子量神经丝蛋白(NF-L)的体外组装

CORC > 北京大学 > 生命科学学院

	低分子量神经丝蛋白(NF-L)的体外组装; The assembly of low molecular weight neurofilament protein (NF-L) in vitro
	佟向军 ; 陈建国 ; 翟中和
刊名	实验生物学报
	1998
关键词	神经丝低分子量神经丝蛋白NF-L 中等分子量神经丝蛋白NF-M 高分子量神经丝蛋白NF-H 重组装亚丝
英文摘要	利用超速离心和离子交换层析技术,从牛脊髓中分离纯化了神经丝蛋白三组分:NF-L,NF-M和NF-H。应用电镜负染色和金属投影方法研究神经丝的形态结构与NF-L的体外组装,结果表明:神经丝由10nm的核心纤维与外周的丝状突起组成;在近似生理条件下,NF-L可在60min内组装成10nm纤维,纤维由4根亚丝缠绕而成;在碱性缓冲液中,NaCl能促进NF-L装配成短纤维,这种10nm的短纤维无法连接成长纤维。; Neurofilaments were isolated from bovine spinal cords by ultra-speed centrifugation and examined by negative staining. The neurofilament triplet proteins: NF-L, NF-M and NF-H were purified by DE-52 anion exchange chromatography in the presence of 6 mol/L urea. The reassembly of NF-L under controlled conditions was studied. NF-L can reassemble into 10 nm width filaments within 60 minutes at physiological condition of around 0.15 mol/L NaCl, 2 mmol/L MgCl2, neutral pH(pH 6.8) and 37 degrees C. In 6 mol/L urea, NF-L was examined as 12 nm-diameter particle by low angle rotary shadowing. When dialyzed against reassembly buffer for 20 minutes, some irregular filaments were formed. Further dialyzed for another 40 minutes, the long smooth filaments appeared. Some filaments were unraveled at the end regions, where existed 2-4 subfilaments. Four subfilaments were more often observed. That is to say, the 10 nm-width filament was composed of 4 subfilaments. While dialyzed against the alkaline buffer containing 0.15 mol/L NaCl, NF-L reconstituted into 45-180 nm-long, 10 nm-width filaments, which were not able to elongate into long filaments.; PubMed; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 0; 03; 223-231; 31
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/45629]
专题	生命科学学院
推荐引用方式 GB/T 7714	佟向军,陈建国,翟中和. 低分子量神经丝蛋白(NF-L)的体外组装, The assembly of low molecular weight neurofilament protein (NF-L) in vitro[J]. 实验生物学报,1998.
APA	佟向军,陈建国,&翟中和.(1998).低分子量神经丝蛋白(NF-L)的体外组装.实验生物学报.
MLA	佟向军,et al."低分子量神经丝蛋白(NF-L)的体外组装".实验生物学报 (1998).