Exploring tight junction alteration using double fluorescent probe   combination of lanthanide complex with gold nanoclusters

doi:10.1038/srep32218

CORC > 北京大学 > 化学与分子工程学院

	Exploring tight junction alteration using double fluorescent probe combination of lanthanide complex with gold nanoclusters
	Wang, Xinyi ; Wang, Na ; Yuan, Lan ; Li, Na ; Wang, Junxia ; Yang, Xiaoda
刊名	SCIENTIFIC REPORTS
	2016
关键词	BLOOD-BRAIN-BARRIER CELL-CULTURE MODEL TRANSPORT-PROPERTIES ADHESION MOLECULES INTESTINAL BARRIER EPITHELIAL-CELLS PERMEABILITY PERMEATION CHITOSAN DRUGS
DOI	10.1038/srep32218
英文摘要	Tight junctions play a key role in restricting or regulating passage of liquids, ions and large solutes through various biological barriers by the paracellular route. Changes in paracellular permeation indicate alteration of the tight junction. However, it is very difficult to obtain the structural change information by measuring paracellular flux based on transepithelial electrical resistance or using fluorescein-labeled dextrans. Here we show that the BSA and GSH stabilized gold nanoclusters exhibit marginal cytotoxicity and pass through the MDCK monolayer exclusively through the paracellular pathway. We propose a double fluorescence probe strategy, the combination of a proven paracellular indicator (europium complex) with fluorescent gold nanoclusters. We calculate changes of structural parameters in tight junctions based on determination of the diffusion coefficients of the probes. Two different types of tight junction openers are used to validate our strategy. Results show that EDTA disrupts tight junction structures and induces large and smooth paracellular pore paths with an average radius of 17 nm, but vanadyl complexes induce paths with the radius of 6 nm. The work suggests that the double fluorescence probe strategy is a useful and convenient approach for in vitro investigation of tight junction structural alternations caused by pharmacological or pathological events.; National Natural Science Foundation of China [21271012, 31571025]; SCI(E); PubMed; ARTICLE; xyang@bjmu.edu.cn; 32218; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/491527]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Xinyi,Wang, Na,Yuan, Lan,et al. Exploring tight junction alteration using double fluorescent probe combination of lanthanide complex with gold nanoclusters[J]. SCIENTIFIC REPORTS,2016.
APA	Wang, Xinyi,Wang, Na,Yuan, Lan,Li, Na,Wang, Junxia,&Yang, Xiaoda.(2016).Exploring tight junction alteration using double fluorescent probe combination of lanthanide complex with gold nanoclusters.SCIENTIFIC REPORTS.
MLA	Wang, Xinyi,et al."Exploring tight junction alteration using double fluorescent probe combination of lanthanide complex with gold nanoclusters".SCIENTIFIC REPORTS (2016).