Investigation and application of photochemically induced direct UV   detection of low or non-UV absorbing compounds by capillary   electrophoresis

doi:10.1016/j.talanta.2016.10.046

CORC > 北京大学 > 化学与分子工程学院

	Investigation and application of photochemically induced direct UV detection of low or non-UV absorbing compounds by capillary electrophoresis
	Xie, Xia ; Chang, Fengxia ; Li, Xin ; Li, Meixian ; Zhu, Zhiwei
刊名	TALANTA
	2017
关键词	Capillary electrophoresis Photolysis Low or non-UV absorbing compounds Amino acid UV detection MICELLAR ELECTROKINETIC CHROMATOGRAPHY AMINO-ACIDS LIQUID-CHROMATOGRAPHY ZONE-ELECTROPHORESIS MASS-SPECTROMETRY SEPARATION DERIVATIZATION PHOTOOXIDATION DERIVATIVES PHOTOLYSIS
DOI	10.1016/j.talanta.2016.10.046
英文摘要	Some low or non-UV absorbing compounds like amino acids might be accessible to direct UV detection by capillary electrophoresis (CE), due to the photochemical reaction in the detection window of the separation capillary under extremely strong alkaline conditions. However, with regards to the photochemical reaction procedure and the influencing factors in CE, no comprehensive studies have been done. Herein, two strategies were applied to investigate the photochemical reaction mechanism including the introduction of an additional UV lamp and the utilization of driving pressure. The former confirmed the occurrence of photolysis, while the latter solved the interference of electroosmotic flow (EOF). Furthermore, the online photochemical,reaction and online preconcentration technique were combined to develop a rapid, simple and sensitive method for determination of seven essential amino acids (valine, leucine, phenylalanine, methionine, tryptophan, threonine and lysine). Eventually, the developed method was successfully applied to the analysis of real samples with good reproducibility and reliability. This novel and simple method, based on the photochemical reactions occurring in the detection window and coupling with online preconcentration techniques, shows a great potential for the rapid and sensitive detection of low or non-UV absorbing compounds.; National Natural Science Foundation of China (NSFC) [21475002, 21335001, 21175006]; SCI(E); ARTICLE; 362-367; 162
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/476532]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xie, Xia,Chang, Fengxia,Li, Xin,et al. Investigation and application of photochemically induced direct UV detection of low or non-UV absorbing compounds by capillary electrophoresis[J]. TALANTA,2017.
APA	Xie, Xia,Chang, Fengxia,Li, Xin,Li, Meixian,&Zhu, Zhiwei.(2017).Investigation and application of photochemically induced direct UV detection of low or non-UV absorbing compounds by capillary electrophoresis.TALANTA.
MLA	Xie, Xia,et al."Investigation and application of photochemically induced direct UV detection of low or non-UV absorbing compounds by capillary electrophoresis".TALANTA (2017).