Epitranscriptome sequencing technologies: decoding RNA modifications

doi:10.1038/NMETH.4110

CORC > 北京大学 > 化学与分子工程学院

	Epitranscriptome sequencing technologies: decoding RNA modifications
	Li, Xiaoyu ; Xiong, Xushen ; Yi, Chengqi
刊名	NATURE METHODS
	2017
关键词	DYNAMIC N-1-METHYLADENOSINE METHYLOME SINGLE-NUCLEOTIDE-RESOLUTION MESSENGER-RNA HUMAN TRANSCRIPTOME WIDESPREAD RNA EDITING SITES RIBOSOMAL-RNA GENE-EXPRESSION NUCLEAR-RNA MAPPING REVEALS
DOI	10.1038/NMETH.4110
英文摘要	In recent years, major breakthroughs in RNA-modification-mediated regulation of gene expression have been made, leading to the emerging field of epitranscriptomics. Our understanding of the distribution, regulation and function of these dynamic RNA modifications is based on sequencing technologies. In this Review, we focus on the major mRNA modifications in the transcriptome of eukaryotic cells: N-6-methyladenosine, N-6,2'-0-dimethyladenosine, 5-methylcytidine, 5-hydroxylmethylcytidine, inosine, pseudouridine and N-1-methyladenosine. We discuss the sequencing technologies used to profile these epitranscriptomic marks, including scale, resolution, quantitative feature, pre-enrichment capability and the corresponding bioinformatics tools. We also discuss the challenges of epitranscriptome profiling and highlight the prospect of future detection tools. We aim to guide the choice of different detection methods and inspire new ideas in RNA biology.; National Basic Research Foundation of China [MOST2016YFC0900300, 2014CB964900]; National Natural Science Foundation of China [21522201]; SCI(E); REVIEW; 1; 23-31; 14
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/476495]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, Xiaoyu,Xiong, Xushen,Yi, Chengqi. Epitranscriptome sequencing technologies: decoding RNA modifications[J]. NATURE METHODS,2017.
APA	Li, Xiaoyu,Xiong, Xushen,&Yi, Chengqi.(2017).Epitranscriptome sequencing technologies: decoding RNA modifications.NATURE METHODS.
MLA	Li, Xiaoyu,et al."Epitranscriptome sequencing technologies: decoding RNA modifications".NATURE METHODS (2017).