Investigation of the interactions between methylene blue and   intramolecular G-quadruplexes: an explicit distinction in   electrochemical behavior

doi:10.1039/c7an00083a

CORC > 北京大学 > 化学与分子工程学院

	Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior
	Cao, Ting ; Zhang, Fang-Ting ; Cai, Liang-Yuan ; Zhou, Ying-Lin ; Buurma, Niklaas J. ; Zhang, Xin-Xiang
刊名	ANALYST
	2017
关键词	HUMAN TELOMERIC DNA STRUCTURAL BASIS BINDING LIGAND PROBE DRUG VISUALIZATION OPTIMIZATION COMPLEXES BIOSENSOR
DOI	10.1039/c7an00083a
英文摘要	G-quadruplex sequences exist in eukaryotic organisms and prokaryotes, and the investigation of the interactions between G-quadruplexes and small molecule ligands is important for gene therapy, biosensor fabrication, fluorescence imaging and so on. Here, we investigated the behaviour of methylene blue (MB), an electroactive molecule, in the presence of different intramolecular G-quadruplexes by an electrochemical method using a miniaturized electrochemical device based on its intrinsic electrochemical properties. Although the effects of MB on different intramolecular G-quadruplex structures are not obvious by circular dichroism spectroscopy, distinct differences in the binding affinities of MB with different intramolecular G-quadruplexes were quickly and easily observed by an electrochemical technique. At the same time, for the human telomerase G-rich sequence (HT), the diffusion current of MB changed sensitively under different ionic conditions due to the formation of different conformations of HT, which indicated that our electrochemical method has the potential to study the influence of metal ions on the conformations of the G-quadruplexes with simplicity, rapid response and low cost. From all these, a new stacking mechanism and rule were obtained, which were also validated by docking studies and isothermal titration calorimetry (ITC).; National Natural Science Foundation of China [21675004, 21575005, 21275009]; SCI(E); ARTICLE; 6; 987-993; 142
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/476139]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Cao, Ting,Zhang, Fang-Ting,Cai, Liang-Yuan,et al. Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior[J]. ANALYST,2017.
APA	Cao, Ting,Zhang, Fang-Ting,Cai, Liang-Yuan,Zhou, Ying-Lin,Buurma, Niklaas J.,&Zhang, Xin-Xiang.(2017).Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior.ANALYST.
MLA	Cao, Ting,et al."Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior".ANALYST (2017).