Structural and mechanistic study of the cysteine oxidation-mediated   induction of the Escherichia coli MarR regulator

doi:10.1016/j.tet.2017.05.039

CORC > 北京大学 > 化学与分子工程学院

	Structural and mechanistic study of the cysteine oxidation-mediated induction of the Escherichia coli MarR regulator
	Zhu, Rongfeng ; Hao, Ziyang ; Lou, Hubing ; Song, Yanqun ; Zhao, Jingyi ; Zhu, Jiuhe ; Chen, Peng R.
刊名	TETRAHEDRON
	2017
关键词	Multiple antibiotic resistance Transcription factors Cysteine oxidation X-ray crystallography Fluorescent reporter MULTIPLE ANTIBIOTIC-RESISTANCE GREEN FLUORESCENT PROTEINS DNA-BINDING TRANSCRIPTIONAL REGULATORS NEGATIVE REGULATOR FAMILY REFINEMENT INTERFACE MOLECULES SUITE
DOI	10.1016/j.tet.2017.05.039
英文摘要	Multiple antibiotic resistance regulator (MarR) family proteins are widely conserved transcription factors that are important for regulating bacterial resistance to various stresses. Escherichia call MarR, the prototype member of this family, has recently been shown to undergo interdimer disulfide bond formation via a unique cysteine residue (Cys80) that ultimately triggered MarR's dissociation from its promoter DNA. However, these structural studies were performed with cysteine mutants while the structure of wild type MarR remains uncharacterized. Here we report the crystal structure of wild type MarR in the absence of DNA, which further revealed the roles of cysteine residues in MarR's transcriptional regulation. In addition, we developed a circularly permuted yellow fluorescent protein (cpYFP)based environmental-sensitive fluorescent reporter for in situ detection of the DNA-binding induced conformational change on MarR, which verified the induction mechanism of this prototypical MarR family protein. This strategy might potentially be applicable for monitoring local conformational change within diverse protein structures. (C) 2017 Elsevier Ltd. All rights reserved.; National Natural Science Foundation of China [21432002, 21521003]; National Key Research and Development Program [2016YFA0501500]; SCI(E); ARTICLE; 26; 3714-3719; 73
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472614]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhu, Rongfeng,Hao, Ziyang,Lou, Hubing,et al. Structural and mechanistic study of the cysteine oxidation-mediated induction of the Escherichia coli MarR regulator[J]. TETRAHEDRON,2017.
APA	Zhu, Rongfeng.,Hao, Ziyang.,Lou, Hubing.,Song, Yanqun.,Zhao, Jingyi.,...&Chen, Peng R..(2017).Structural and mechanistic study of the cysteine oxidation-mediated induction of the Escherichia coli MarR regulator.TETRAHEDRON.
MLA	Zhu, Rongfeng,et al."Structural and mechanistic study of the cysteine oxidation-mediated induction of the Escherichia coli MarR regulator".TETRAHEDRON (2017).