Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic,   transcriptomic and phosphoproteomic analyses in a null mutant of the PKA   catalytic subunit in Candida albicans

doi:10.1111/mmi.13681

CORC > 北京大学 > 化学与分子工程学院

	Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in Candida albicans
	Cao, Chengjun ; Wu, Mei ; Bing, Jian ; Tao, Li ; Ding, Xuefen ; Liu, Xiaoyun ; Huang, Guanghua
刊名	MOLECULAR MICROBIOLOGY
	2017
关键词	PROTEIN-KINASE-A PSEUDOHYPHAL DIFFERENTIATION SACCHAROMYCES-CEREVISIAE CRYPTOCOCCUS-NEOFORMANS HYPHAL MORPHOGENESIS SEXUAL DEVELOPMENT GENE DELETION FISSION YEAST VIRULENCE PHOSPHORYLATION
DOI	10.1111/mmi.13681
英文摘要	The conserved cAMP-dependent protein kinase (PKA) plays critical roles in the regulation of morphological transitions and virulence in the human fungal pathogen Candida albicans. It has long been thought that the PKA catalytic subunit is essential for cell viability in this fungus. Paradoxically, the single adenylyl cyclase-encoding gene, CYR1, which is required for the production of cAMP in C. albicans, is not essential for cell growth. Here, a double mutant of TPK1 and TPK2 (tpk2/tpk2 tpk1/tpk1, t2t1), which encode two isoforms of the PKA catalytic subunit was successfully generated, suggesting that this subunit is not essential for cell viability. Inactivation of the PKA catalytic subunit blocked filamentation and dramatically attenuated white-to-opaque switching, but promoted sexual mating. Comparative transcriptomic analyses demonstrated that the t2t1 and cyr1/cyr1 mutants exhibited similar global gene expression profiles. Compared with the WT strain, the general transcriptional activity and metabolism were significantly decreased in both the t2t1 and cyr1/cyr1 mutants. Using combined phosphoproteomic and bioinformatic analyses, we identified 181 potential PKA phosphorylation targets, which represent 148 unique proteins involved in a wide spectrum of biological processes. The study sheds new insights into the global regulatory features of the cAMP/PKA pathway in C. albicans.; Chinese National Natural Science Foundation [31625002, 31370175, 81322026]; SCI(E); ARTICLE; 1; 46-64; 105
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472480]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Cao, Chengjun,Wu, Mei,Bing, Jian,et al. Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in Candida albicans[J]. MOLECULAR MICROBIOLOGY,2017.
APA	Cao, Chengjun.,Wu, Mei.,Bing, Jian.,Tao, Li.,Ding, Xuefen.,...&Huang, Guanghua.(2017).Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in Candida albicans.MOLECULAR MICROBIOLOGY.
MLA	Cao, Chengjun,et al."Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in Candida albicans".MOLECULAR MICROBIOLOGY (2017).