Structural characterization of the DNA-binding mechanism underlying the   copper(II)-sensing MarR transcriptional regulator

doi:10.1007/s00775-017-1442-7

CORC > 北京大学 > 化学与分子工程学院

	Structural characterization of the DNA-binding mechanism underlying the copper(II)-sensing MarR transcriptional regulator
	Zhu, Rongfeng ; Hao, Ziyang ; Lou, Hubing ; Song, Yanqun ; Zhao, Jingyi ; Chen, Yuqing ; Zhu, Jiuhe ; Chen, Peng R.
刊名	8th Asian Biological Inorganic Chemistry Conference (AsBIC)
	2017
关键词	Multiple antibiotic resistance Transcription regulation Protein-DNA interactions X-ray crystallography MULTIPLE-ANTIBIOTIC-RESISTANCE ESCHERICHIA-COLI CRYSTAL-STRUCTURE MUTATIONAL ANALYSIS NEGATIVE REGULATOR FAMILY REPRESSOR INTERFACE REVEALS PROTEIN
DOI	10.1007/s00775-017-1442-7
英文摘要	Multiple antibiotic resistance regulator (MarR) family proteins are widely conserved transcription factors that control bacterial resistance to antibiotics, environmental stresses, as well as the regulation of virulence determinants. Escherichia coli MarR, the prototype member of this family, has recently been shown to undergo copper(II)-catalyzed inter-dimer disulfide bond formation via a unique cysteine residue (Cys80) residing in its DNA-binding domain. However, despite extensive structural characterization of the MarR family proteins, the structural mechanism for DNA binding of this copper(II)-sensing MarR factor remains elusive. Here, we report the crystal structures of DNA-bound forms of MarR, which revealed a unique, concerted generation of two new helix-loop-helix motifs that facilitated MarR's DNA binding. Structural analysis and electrophoretic mobility shift assays (EMSA) show that the flexibility of Gly116 in the center of helix alpha 5 and the extensive hydrogen-bonding interactions at the N-terminus of helix alpha 1 together assist the reorientation of the wHTH domains and stabilize MarR's DNA-bound conformation.; National Natural Science Foundation of China [21225206, 21432002, 21521003]; National Key Research and Development Program [2016YFA0501500]; SCI(E); ARTICLE; 5; 685-693; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472413]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhu, Rongfeng,Hao, Ziyang,Lou, Hubing,et al. Structural characterization of the DNA-binding mechanism underlying the copper(II)-sensing MarR transcriptional regulator[J]. 8th Asian Biological Inorganic Chemistry Conference (AsBIC),2017.
APA	Zhu, Rongfeng.,Hao, Ziyang.,Lou, Hubing.,Song, Yanqun.,Zhao, Jingyi.,...&Chen, Peng R..(2017).Structural characterization of the DNA-binding mechanism underlying the copper(II)-sensing MarR transcriptional regulator.8th Asian Biological Inorganic Chemistry Conference (AsBIC).
MLA	Zhu, Rongfeng,et al."Structural characterization of the DNA-binding mechanism underlying the copper(II)-sensing MarR transcriptional regulator".8th Asian Biological Inorganic Chemistry Conference (AsBIC) (2017).