Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana

doi:10.1002/cbic.201700069

CORC > 北京大学 > 化学与分子工程学院

	Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana
	Zhu, Yuntao ; Chen, Xing
刊名	CHEMBIOCHEM
	2017
关键词	Arabidopsis thaliana cell wall polysaccharide click chemistry glycoconjugates metabolic glycan labeling plant glycobiology PLANT-CELL WALL GLCNAC-MODIFIED PROTEINS DEVELOPING ZEBRAFISH CHEMICAL REPORTERS SIALIC-ACID IN-VIVO GROWTH BIOSYNTHESIS PROBES IDENTIFICATION
DOI	10.1002/cbic.201700069
英文摘要	Metabolic glycan labeling (MGL) has gained wide utility and has become a useful tool for probing glycosylation in living systems. For the past three decades, the development and application of MGL have mostly focused on animal glycosylation. Recently, exploiting MGL for studying plant glycosylation has gained interest. Here, we describe a systematic evaluation of MGL for fluorescence imaging of root glycans in Arabidopsis thaliana. Nineteen monosaccharide analogues containing a bioorthogonal group (azide, alkyne, or cyclopropene) were synthesized and evaluated for metabolic incorporation into root glycans. Among these unnatural sugars, 14 (including three new compounds) were evaluated in plants for the first time. Our results showed that five unnatural sugars metabolically labeled root glycans efficiently, and enabled fluorescence imaging by bioorthogonal conjugation with fluorophores. We optimized the experimental procedures for MGL in Arabidopsis. Finally, distinct distribution patterns of the newly synthesized glycans were observed along the root developmental zones, thus indicating regulated biosynthesis of glycans during root development. We envision that MGL will find broad applications in plant glycobiology.; National Natural Science Foundation of China [21425204, 21521003, 21672013]; National Key Research and Development Projects [2016YFA0501500]; SCI(E); ARTICLE; 13,SI; 1286-1296; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472265]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Zhu, Yuntao,Chen, Xing. Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana[J]. CHEMBIOCHEM,2017.
APA	Zhu, Yuntao,&Chen, Xing.(2017).Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana.CHEMBIOCHEM.
MLA	Zhu, Yuntao,et al."Expanding the Scope of Metabolic Glycan Labeling in Arabidopsis thaliana".CHEMBIOCHEM (2017).