Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the   Mouse Gut

doi:10.1021/acs.biochem.7b00539

CORC > 北京大学 > 化学与分子工程学院

	Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut
	Wang, Wei ; Zhu, Yuntao ; Chen, Xing
刊名	BIOCHEMISTRY
	2017
关键词	SPATIAL-ORGANIZATION TERMINAL ALKYNES BACTERIA PEPTIDOGLYCAN CORE AZIDES
DOI	10.1021/acs.biochem.7b00539
英文摘要	The diverse gut microbial communities are crucial for host health. How the interactions between microbial communities and between host and microbes influence the host, however, is not well understood. To facilitate gut microbiota research, selective imaging of specific groups of microbiotas in the gut is of great utility but remains technically challenging. Here we present a chemical approach that enables selective imaging of Gram-negative and Gram-positive microbiotas in the mouse gut by exploiting their distinctive cell wall components. Cell selective labeling is achieved by the combined use of metabolic labeling of Gram-negative bacterial lipopolysaccharides with a clickable azidosugar and direct labeling of Grain-positive bacteria with a vancomycin-derivatized fluorescent probe. We demonstrated this strategy by two-color fluorescence imaging of Gram-negative and Grain-positive gut microbiotas in the mouse intestines. This chemical, method should be broadly applicable to different gut microbiota research fields and other bacterial communities studied in microbiology.; National Natural Science Foundation of China [21672013, 21425204, 21521003]; National Key Research and Development Projects [2016YFA0501500]; SCI(E); ARTICLE; 30; 3889-3893; 56
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471774]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Wang, Wei,Zhu, Yuntao,Chen, Xing. Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut[J]. BIOCHEMISTRY,2017.
APA	Wang, Wei,Zhu, Yuntao,&Chen, Xing.(2017).Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut.BIOCHEMISTRY.
MLA	Wang, Wei,et al."Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut".BIOCHEMISTRY (2017).