Dissection of Kinase Isoforms through Orthogonal and Chemical Inducible   Signaling Cascades

doi:10.1002/cbic.201700255

CORC > 北京大学 > 化学与分子工程学院

	Dissection of Kinase Isoforms through Orthogonal and Chemical Inducible Signaling Cascades
	Zheng, Siqi ; Fan, Xinyuan ; Wang, Jie ; Zhao, Jingyi ; Chen, Peng R.
刊名	CHEMBIOCHEM
	2017
关键词	bacterial effector proteins cycloaddition decaging kinases protein activation signaling pathways MAMMALIAN-CELLS PROTEIN-KINASES LIVING CELLS MEK1 GROWTH ACTIVATION PATHWAYS
DOI	10.1002/cbic.201700255
英文摘要	Interference from endogenous signaling enzymes represents a major hurdle for building orthogonal signaling cascades inside cells, particularly among closely related isoforms within an enzyme family. Here, we employed a genetically encoded chemical decaging strategy to build orthogonally activated kinase isoforms, with the endogenous counterparts temporally disabled by an extracellularly delivered bacterial effector. This approach eliminated any potential interference from other kinase isoforms as well as endogenous kinases, which allowed the specific, gain-of-function report of mitogen-activated protein kinase kinase1 (MEK1) activity as opposed to MEK2 with high temporal resolution. Our study dissected the distinct enzymatic activity, feedback regulation and signal outputs between these closely related kinase isoforms.; National Key Research and Development Program of China [2016YFA0501500]; National Natural Science Foundation of China [21521003, 21432002]; SCI(E); ARTICLE; 16; 1593-1598; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471440]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Zheng, Siqi,Fan, Xinyuan,Wang, Jie,et al. Dissection of Kinase Isoforms through Orthogonal and Chemical Inducible Signaling Cascades[J]. CHEMBIOCHEM,2017.
APA	Zheng, Siqi,Fan, Xinyuan,Wang, Jie,Zhao, Jingyi,&Chen, Peng R..(2017).Dissection of Kinase Isoforms through Orthogonal and Chemical Inducible Signaling Cascades.CHEMBIOCHEM.
MLA	Zheng, Siqi,et al."Dissection of Kinase Isoforms through Orthogonal and Chemical Inducible Signaling Cascades".CHEMBIOCHEM (2017).