Temporal Regulation of a Salmonella Typhimurium Virulence Factor by the   Transcriptional Regulator YdcR

doi:10.1074/mcp.M117.068296

CORC > 北京大学 > 化学与分子工程学院

	Temporal Regulation of a Salmonella Typhimurium Virulence Factor by the Transcriptional Regulator YdcR
	Liu, Yanhua ; Liu, Qian ; Qi, Linlu ; Ding, Tao ; Wang, Zhen ; Fu, Jiaqi ; Hu, Mo ; Li, Min ; Song, Jeongmin ; Liu, Xiaoyun
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2017
关键词	HOST EPITHELIAL-CELLS CHROMOSOMAL GENES BACILLUS-SUBTILIS EXPRESSION PROTEIN FAMILY DOMAIN MEMBER GABR SLYA
DOI	10.1074/mcp.M117.068296
英文摘要	We previously examined Salmonella proteome within infected host cells and found differential expression of many proteins with defined functional roles such as metabolism or virulence. However, the precise roles of other altered proteins in Salmonella pathogenesis are largely unknown. A putative transcriptional regulator, YdcR, was highly induced intracellularly whereas barely expressed in vitro, implicating potential relevance to bacterial infection. To unveil its physiological functions, we exploited quantitative proteomics of intracellular Salmonella and found that genetic ablation of ydcR resulted in severe repression of SrfN, a known virulence factor. Immunoblotting, qRT-PCR, and beta-galactosidase assays further demonstrate YdcR-dependent transcription and expression of srfN. Moreover, we found physical interaction of YdcR with the promoter region of srfN, suggesting direct activation of its transcription. Importantly, a Salmonella mutant lacking ydcR was markedly attenuated in a mouse model of infection. Our findings reveal that YdcR temporally regulates the virulence factor SrfN during infection, thus contributing to Salmonella pathogenesis. Our work also highlights the utility of combining quantitative proteomics and bacterial genetics for uncovering the functional roles of transcription factors and likely other uncharacterized proteins as well.; National Natural Science Foundation of China [21475005, 21622501]; Thousand Young Talents program of the Chinese government; SCI(E); ARTICLE; 9; 1683-1693; 16
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471179]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Yanhua,Liu, Qian,Qi, Linlu,et al. Temporal Regulation of a Salmonella Typhimurium Virulence Factor by the Transcriptional Regulator YdcR[J]. MOLECULAR & CELLULAR PROTEOMICS,2017.
APA	Liu, Yanhua.,Liu, Qian.,Qi, Linlu.,Ding, Tao.,Wang, Zhen.,...&Liu, Xiaoyun.(2017).Temporal Regulation of a Salmonella Typhimurium Virulence Factor by the Transcriptional Regulator YdcR.MOLECULAR & CELLULAR PROTEOMICS.
MLA	Liu, Yanhua,et al."Temporal Regulation of a Salmonella Typhimurium Virulence Factor by the Transcriptional Regulator YdcR".MOLECULAR & CELLULAR PROTEOMICS (2017).