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	Biocompatible bright YVO4:Eu nanoparticles as versatile optical bioprobes
	Shen, Jie ; Sun, Ling-Dong ; Zhu, Jia-Dan ; Wei, Liu-He ; Sun, Hong-Fang ; Yan, Chun-Hua
	2010
英文摘要	Lanthanide nanoparticles (NPs), characterized by their large Stokes shifts, narrow emission bands and high photochemical stability, are promising candidates for optical probes in biological research. As a typical red emission material, Eu3+-doped YVO4 is investigated in pursuit of an integral methodology for Ln-based bioprobes. Assisted with phosphino- polyacrylic acid, YVO4:Eu NPs with a luminescent quantum yield of ca. 54% are synthesized via a one-pot hydrothermal reaction. The obtained NPs exhibit good water/buffer stability and feasible biomodification benefiting from the surface carboxylic acid groups. However, having an inorganic core of ca. 20 nm, these NPs do not affect the conformation of surface conjugated proteins (bovine serum albumin), as confirmed with the circular dichroism spectrum. A specific recognition protocol is demonstrated by biotinated YVO4:Eu NPs in quantitative fluoroimmunoassay and microchip assay. Furthermore, the biocompatibility of the Ln NPs, which is an important aspect towards biological application, is demonstrated with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay. Cell imaging studies conducted with spectral mapping technique and confocal fluorescent microscopy demonstrate the YVO 4:Eu NPs to be a type of competitive luminescent probes for in vitro applications. ? 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.; EI; 21; 3708-3714; 20
语种	英语
DOI标识	10.1002/adfm.201001264
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/461781]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Shen, Jie,Sun, Ling-Dong,Zhu, Jia-Dan,et al. Biocompatible bright YVO4:Eu nanoparticles as versatile optical bioprobes. 2010-01-01.

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