Palladium-Triggered Chemical Rescue of Intracellular Proteins via   Genetically Encoded Allene-Caged Tyrosine

doi:10.1021/jacs.6b08933

CORC > 北京大学 > 化学与分子工程学院

	Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine
	Wang, Jie ; Zheng, Siqi ; Liu, Yanjun ; Zhang, Zhaoyue ; Lin, Zhi ; Li, Jiaofeng ; Zhang, Gong ; Wang, Xin ; Li, Jie ; Chen, Peng R.
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2016
关键词	RNA SYNTHETASE MUTANT ANTHRAX LETHAL FACTOR ACTIVE-SITE TYROSINE DNA-POLYMERASE-I CRYSTAL-STRUCTURE LIVING CELLS BIOORTHOGONAL CHEMISTRY STRUCTURAL BASIS MECHANISM CLEAVAGE
DOI	10.1021/jacs.6b08933
英文摘要	Chemical de-caging has emerged as an attractive strategy for gain-of-function study of proteins via small-molecule reagents. The previously reported chemical de-caging reactions have been largely centered on liberating the side chain of lysine on a given protein. Herein, we developed an allene-based caging moiety and the corresponding palladium de-caging reagents for chemical rescue of tyrosine (Tyr) activity on intracellular proteins. This bioorthogonal de-caging pair has been successfully applied to unmask enzymatic Tyr sites (e.g., Y671 on Taq polymerase and Y728 on Anthrax lethal factor) as well as the post-translational Tyr modification site (Y416 on Src kinase) in vitro and in living cells. Our strategy provides a general platform for chemical rescue of Tyr-dependent protein activity inside cells.; National Natural Science Foundation of China [21225206, 21521003, 21432002]; National Basic Research Program of China [2012-CB917301]; SCI(E); ARTICLE; pengchen@pku.edu.cn; 46; 15118-15121; 138
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/458383]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Wang, Jie,Zheng, Siqi,Liu, Yanjun,et al. Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2016.
APA	Wang, Jie.,Zheng, Siqi.,Liu, Yanjun.,Zhang, Zhaoyue.,Lin, Zhi.,...&Chen, Peng R..(2016).Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY.
MLA	Wang, Jie,et al."Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016).