Immobilization-free DNA-based homogeneous electrochemical biosensors

doi:10.1016/j.trac.2016.08.012

CORC > 北京大学 > 化学与分子工程学院

	Immobilization-free DNA-based homogeneous electrochemical biosensors
	Zhang, Fang-Ting ; Cai, Liang-Yuan ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	TRAC-TRENDS IN ANALYTICAL CHEMISTRY
	2016
关键词	DNA Biosensors Immobilization-free Homogeneous Electrochemistry ROLLING-CIRCLE AMPLIFICATION HEMIN/G-QUADRUPLEX DNAZYME SINGLE-STRANDED-DNA TARGET RECYCLING AMPLIFICATION HYBRIDIZATION CHAIN-REACTION SEQUENCE-SPECIFIC DETECTION STRUCTURE-SWITCHING DNA REDUCED GRAPHENE OXIDE LABEL-FREE NUCLEIC-ACID
DOI	10.1016/j.trac.2016.08.012
英文摘要	DNA is an excellent biomaterial for building biosensors utilizing diverse DNA interactions with a broad range of biomolecules and chemical compounds. DNA-based electrochemical biosensors have been successfully used in numerous applications with advantages of rapid detection, high sensitivity and low cost. Typical DNA-based electrochemical biosensors require an immobilization step of DNA probe as the biological recognition element, which always suffers from time-consuming and labor-intensive electrode pretreatment and immobilization process, expensive chemical modification, lower reactivity, and complicated operation. Immobilization-free DNA-based electrochemical biosensors can provide powerful complementary tools and novel design strategies for traditional electrochemical DNA biosensors. They would be advantageous in some ways, such as low cost, easy operation, and time saving. More importantly, the homogeneous reaction could increase the reliability and reproducibility of electrochemical sensor without the immobilization step. This review introduces multiple immobilization-free strategies for developing DNA-based homogeneous electrochemical biosensors, aiming at compensating for the deficiencies of heterogeneous modes. The main signal readout modes and signal enhancement strategies will be discussed separately. Additionally, the burgeoning applications combined with miniaturization of electrochemical devices will be highlighted. (C) 2016 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21275009, 21575005]; SCI(E); EI; REVIEW; zhouyl@pku.edu.cn; zxx@pku.edu.cn; 17-32; 85
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/449958]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Fang-Ting,Cai, Liang-Yuan,Zhou, Ying-Lin,et al. Immobilization-free DNA-based homogeneous electrochemical biosensors[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2016.
APA	Zhang, Fang-Ting,Cai, Liang-Yuan,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2016).Immobilization-free DNA-based homogeneous electrochemical biosensors.TRAC-TRENDS IN ANALYTICAL CHEMISTRY.
MLA	Zhang, Fang-Ting,et al."Immobilization-free DNA-based homogeneous electrochemical biosensors".TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2016).