The formation and characteristics of the i-motif structure within the   promoter of the c-myb proto-oncogene

doi:10.1016/j.jphotobiol.2016.07.035

CORC > 北京大学 > 化学与分子工程学院

	The formation and characteristics of the i-motif structure within the promoter of the c-myb proto-oncogene
	Li, Huihui ; Hai, Jinhui ; Zhou, Jiang ; Yuan, Gu
刊名	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
	2016
关键词	i-Motif C-myb Circular dichroism Electrospray ionization mass spectrometry Conformational switching Molecular crowding G-QUADRUPLEX DNA MASS-SPECTROMETRY CIRCULAR-DICHROISM GENE-EXPRESSION SEQUENCES ONCOGENE BINDING SWITCH OLIGODEOXYNUCLEOTIDES CONFORMATION
DOI	10.1016/j.jphotobiol.2016.07.035
英文摘要	C-myb proto-oncogene is a potential therapeutic target for some human solid tumors and leukemias. A long cytosine -rich sequence, which locates the downstream of the transcription initiation site, is demonstrated to fold into an intramolecular i-motif DNA using electrospray ionization mass spectrometry (ESI-MS) and circular dichroism (CD) spectroscopy. Effects of factors, including the pH value, the number of C:C+ dimers, the concentration of buffer, the molecular crowding condition, and the coexistence of the complementary DNA, on the formation and the structural stability of the i-motif DNA are systematically studied. We have demonstrated that the i-motif folding in the c-myb promoter could be accelerated upon synergistic physiological stimuli including intracellular molecular crowding and low pH values, as well as the large number of the i-motif C:C+ dimers. Meanwhile, various inputs, such as acids/bases and metal ions, have exhibited their abilities in controlling the conformational switch of the c-myb GC -rich DNA. Acidic pH values and the presence of K+ ions can induce the dissociation of the double helix. Our present strategy can greatly extend the potential usages of i-motif DNA molecules with specific sequences as conformational switch-controlled devices. Moreover, this work demonstrates the superiority of CD spectroscopy associated with ESI-MS as a rapid, more cost-effective and sensitive structural change responsive method in the research of DNA conformational switching. (C) 2016 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21372021]; 973 Program [2012CB720600, 2012CB720601]; Natural Science Foundation of the Higher Education Institutions of Jiangsu Province [15KJB150017]; Beijing National Laboratory for Molecular Sciences [20140167]; State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1413]; Priority Academic Program Development of Jiangsu Higher Education Institution; SCI(E); PubMed; ARTICLE; huihuili@njnu.edu.cn; guyuan@pku.edu.cn; 625-632; 162
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/448030]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Huihui,Hai, Jinhui,Zhou, Jiang,et al. The formation and characteristics of the i-motif structure within the promoter of the c-myb proto-oncogene[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2016.
APA	Li, Huihui,Hai, Jinhui,Zhou, Jiang,&Yuan, Gu.(2016).The formation and characteristics of the i-motif structure within the promoter of the c-myb proto-oncogene.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY.
MLA	Li, Huihui,et al."The formation and characteristics of the i-motif structure within the promoter of the c-myb proto-oncogene".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (2016).