Near-Infrared Light Activation of Proteins Inside Living Cells Enabled   by Carbon Nanotube-Mediated Intracellular Delivery

doi:10.1021/acsami.6b00323

CORC > 北京大学 > 化学与分子工程学院

	Near-Infrared Light Activation of Proteins Inside Living Cells Enabled by Carbon Nanotube-Mediated Intracellular Delivery
	Li, He ; Fan, Xinqi ; Chen, Xing
刊名	ACS APPLIED MATERIALS & INTERFACES
	2016
关键词	bionanotechnology spatiotemporal control near-infrared light protein activation protein delivery RIBOSOME-INACTIVATING PROTEINS IN-VIVO PHOTOCHEMICAL INTERNALIZATION PHOTODYNAMIC THERAPY DRUG-DELIVERY CANCER SAPORIN TRANSPORTERS NANOPARTICLES THERAPEUTICS
DOI	10.1021/acsami.6b00323
英文摘要	Light-responsive proteins have been delivered into the cells for controlling intracellular events with high spatial and temporal resolution. However, the choice of wavelength is limited to the UV and visible range; activation of proteins inside the cells using near-infrared (NIR) light, which has better tissue penetration and biocompatibility, remains elusive. Here, we report the development of a single-walled carbon nanotube (SWCNT)-based bifunctional system that enables protein intracellular delivery, followed by NIR activation of the delivered proteins inside the cells. Proteins of interest are conjugated onto SWCNTs via a streptavidin-desthiobiotin (SA-DTB) linkage, where the protein activity is blocked. SWCNTs serve as both a nanocarrier for carrying proteins into the cells and subsequently a NIR sensitizer to photothermally cleave the linkage and release the proteins. The released proteins become active and exert their functions inside the cells. We demonstrated this strategy by intracellular delivery and NIR-triggered nuclear translocation of enhanced green fluorescent protein, and by intracellular delivery and NIR-activation of a therapeutic protein, saporin, in living cells. Furthermore, we showed that proteins conjugated onto SWCNTs via the SA-DTB linkage could be delivered to the tumors, and optically released and activated by using NIR light in living mice.; National Natural Science Foundation of China [21425204]; DuPont; SCI(E); ARTICLE; xingchen@pku.edu.cn; 7; 4500-4507; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/438599]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, He,Fan, Xinqi,Chen, Xing. Near-Infrared Light Activation of Proteins Inside Living Cells Enabled by Carbon Nanotube-Mediated Intracellular Delivery[J]. ACS APPLIED MATERIALS & INTERFACES,2016.
APA	Li, He,Fan, Xinqi,&Chen, Xing.(2016).Near-Infrared Light Activation of Proteins Inside Living Cells Enabled by Carbon Nanotube-Mediated Intracellular Delivery.ACS APPLIED MATERIALS & INTERFACES.
MLA	Li, He,et al."Near-Infrared Light Activation of Proteins Inside Living Cells Enabled by Carbon Nanotube-Mediated Intracellular Delivery".ACS APPLIED MATERIALS & INTERFACES (2016).