Sensitive discrimination of stable mismatched base pairs by an abasic   site modified fluorescent probe and lambda exonuclease

doi:10.1039/c5cc05749c

CORC > 北京大学 > 化学与分子工程学院

	Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease
	Wu, Tongbo ; Xiao, Xianjin ; Gu, Feidan ; Zhao, Meiping
刊名	CHEMICAL COMMUNICATIONS
	2015
关键词	SINGLE-NUCLEOTIDE POLYMORPHISMS SWITCH-ON PROBE MYELOPROLIFERATIVE DISORDERS NUCLEIC-ACIDS GENOMIC DNA JAK2 V617F PCR OLIGONUCLEOTIDE AMPLIFICATION MUTATIONS
DOI	10.1039/c5cc05749c
英文摘要	An abasic site modified fluorescent probe has been developed which enabled the rapid discrimination of stable single mismatched base pairs by lambda exonuclease with remarkably high discrimination factors (447 for T:G and 238 for A:G). This method allowed sensitive detection of single nucleotide variation at very low abundances (0.02-0.05% mutant-to-wild type).; National Natural Science Foundation of China [21375004]; Beijing Natural Science Foundation [2152014]; SCI(E); PubMed; ARTICLE; mpzhao@pku.edu.cn; 98; 17402-17405; 51
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417506]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wu, Tongbo,Xiao, Xianjin,Gu, Feidan,et al. Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease[J]. CHEMICAL COMMUNICATIONS,2015.
APA	Wu, Tongbo,Xiao, Xianjin,Gu, Feidan,&Zhao, Meiping.(2015).Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease.CHEMICAL COMMUNICATIONS.
MLA	Wu, Tongbo,et al."Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease".CHEMICAL COMMUNICATIONS (2015).