Unimolecular Chemically Modified DNA Fluorescent Probe for One-Step   Quantitative Measurement of the Activity of Human Apurinic/Apyrimidinic   Endonuclease 1 in Biological Samples

doi:10.1021/acs.analchem.5b03939

CORC > 北京大学 > 化学与分子工程学院

	Unimolecular Chemically Modified DNA Fluorescent Probe for One-Step Quantitative Measurement of the Activity of Human Apurinic/Apyrimidinic Endonuclease 1 in Biological Samples
	Fang, Simin ; Chen, Lu ; Zhao, Meiping
刊名	ANALYTICAL CHEMISTRY
	2015
关键词	BASE EXCISION-REPAIR ABASIC ENDONUCLEASE BINDING ACTIVITY ENZYME ASSAY PROTEIN AGENTS CELLS REF-1 APE
DOI	10.1021/acs.analchem.5b03939
英文摘要	A novel DNA structure containing a 3' internal-loop modified abasic site has been constructed which enables effective differentiation between apurinic/apyrimidinic endonuclease (APE1) and nonspecific endonuclease (DNase I). When this unique substrate structure is employed, a double-loop frayed-end chimeric fluorescent probe is successfully developed for quantitative measurement of the activity of APE1 in biological samples without the need of additional cleanup or preconcentration steps. The method is simple and rapid and has a single-step with a linear working range between 0.1 and 5.0 U/mL and a lower limit of detection of 0.1 U/mL. It holds great potential in real-time monitoring of the variation of intracellular and extracellular APE1, which will be very useful for further understanding of the DNA repair pathways in different organisms.; NSFC [21375004, 21575008]; Beijing NSF [2152014]; SCI(E); EI; PubMed; ARTICLE; mpzhao@pku.edu.cn; 24; 11952-11956; 87
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/416515]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Fang, Simin,Chen, Lu,Zhao, Meiping. Unimolecular Chemically Modified DNA Fluorescent Probe for One-Step Quantitative Measurement of the Activity of Human Apurinic/Apyrimidinic Endonuclease 1 in Biological Samples[J]. ANALYTICAL CHEMISTRY,2015.
APA	Fang, Simin,Chen, Lu,&Zhao, Meiping.(2015).Unimolecular Chemically Modified DNA Fluorescent Probe for One-Step Quantitative Measurement of the Activity of Human Apurinic/Apyrimidinic Endonuclease 1 in Biological Samples.ANALYTICAL CHEMISTRY.
MLA	Fang, Simin,et al."Unimolecular Chemically Modified DNA Fluorescent Probe for One-Step Quantitative Measurement of the Activity of Human Apurinic/Apyrimidinic Endonuclease 1 in Biological Samples".ANALYTICAL CHEMISTRY (2015).