The genomic sequences near the mir-23b-27b-24-1 cluster form   G-quadruplexes and are selectively bound by the natural alkaloid   tetrandrine

doi:10.1002/rcm.7251

CORC > 北京大学 > 化学与分子工程学院

	The genomic sequences near the mir-23b-27b-24-1 cluster form G-quadruplexes and are selectively bound by the natural alkaloid tetrandrine
	Qi, Yanchao ; Chen, Han ; Tan, Wei ; Li, Yanyan ; Yuan, Gu ; Xu, Ming
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2015
关键词	IONIZATION MASS-SPECTROMETRY RNA G-QUADRUPLEXES NONCOVALENT INTERACTION CIRCULAR-DICHROISM PROMOTER REGION SMALL-MOLECULE RECOGNITION EXPRESSION BINDING DNA
DOI	10.1002/rcm.7251
英文摘要	RATIONALE: Although the microRNAs miR-23b, miR-27b and miR-24 are located in the same cluster, their expressions in various pathological states are not always comparable. By searching the genomic sequence around mir23b-27b-24-1 in rat, we identified three potential G-quadruplex sequences (PQS) which can fold into different types of G-quadruplexes, including parallel or antiparallel. Natural alkaloids, tetrandrine (TET), displayed different binding affinity with the three G-quadruplexes which may potentially regulate the expression of mir23b-27b-24-1 cluster members. METHODS: Both electrospray ionization mass spectrometry (ESI-MS) and circular dichroism (CD) spectroscopy were utilized to detect the formation of G-quadruplexes. Six small molecules were screened by ESI-MS for their binding affinity with three G-quadruplexes, which were evaluated by their IRa values. RESULTS: The results of ESI-MS and CD experiments confirmed the formation of three G-quadruplexes neighboring the mir23b-27b-24-1 cluster; two of them adopted antiparallel G-quadruplexes, another adopted a parallel G-quadruplex. Screening of small molecules by ESI-MS showed tetrandrine had selective binding affinity for the parallel G-quadruplex. G-quadruplex stabilization by tetrandrine was verified by CD variable temperature measurements. CONCLUSIONS: The gene of the mir23b-27b-24-1 cluster harbors three G-quadruplexes with typical sequences and structures. Tetrandrine had a selective binding affinity to the parallel G-quadruplex and stabilized it significantly. Copyright (C) 2015 John Wiley & Sons, Ltd.; Beijing National Laboratory for Molecular Sciences; National Natural Science Foundation of China [91339105]; Natural Science Foundation of Beijing [7142165]; SCI(E); ARTICLE; xuminghi@bjmu.edu.cn; 17; 1611-1616; 29
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/416277]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Qi, Yanchao,Chen, Han,Tan, Wei,et al. The genomic sequences near the mir-23b-27b-24-1 cluster form G-quadruplexes and are selectively bound by the natural alkaloid tetrandrine[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2015.
APA	Qi, Yanchao,Chen, Han,Tan, Wei,Li, Yanyan,Yuan, Gu,&Xu, Ming.(2015).The genomic sequences near the mir-23b-27b-24-1 cluster form G-quadruplexes and are selectively bound by the natural alkaloid tetrandrine.RAPID COMMUNICATIONS IN MASS SPECTROMETRY.
MLA	Qi, Yanchao,et al."The genomic sequences near the mir-23b-27b-24-1 cluster form G-quadruplexes and are selectively bound by the natural alkaloid tetrandrine".RAPID COMMUNICATIONS IN MASS SPECTROMETRY (2015).