Hydrazino-s-triazine based labelling reagents for highly sensitive   glycan analysis via liquid chromatography-electrospray mass spectrometry

doi:10.1016/j.talanta.2015.07.045

CORC > 北京大学 > 化学与分子工程学院

	Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry
	Zhao, Ming-Zhe ; Zhang, Yi-Wei ; Yuan, Fang ; Deng, Yan ; Liu, Jing-Xin ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	TALANTA
	2015
关键词	Glycan Labelling reagent Controllable synthesis Liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) Glycomics profiling ASSISTED-LASER-DESORPTION/IONIZATION N-LINKED OLIGOSACCHARIDES CAPILLARY-ELECTROPHORESIS GLYCOSYLATION PATTERN SERUM IGG IONIZATION MATRIX DERIVATIZATION TIME QUANTIFICATION
DOI	10.1016/j.talanta.2015.07.045
英文摘要	Labelling strategy plays an important role in mass spectrometry (MS) based glycan analysis due to the high hydrophilicity and low ionization efficiency of glycans. Ten hydrazino-s-triazine based labelling reagents were synthesized under facile and controllable conditions for highly sensitive liquid chromatography-electrospray mass spectrometry glycan analysis in this work. Attached to N-glycans through non-reductive reactions, these new labelling reagents were evaluated in aspect of the differently enhanced glycan response to mass spectrometry. Three of the ten labelling reagents demonstrated to be reliable and remarkable for glycan analysis with satisfactory linearity and lowered limits of detection using maltoheptaose (DP7) as model. Furthermore, the most optimal labelling reagent was taken as an example for highly sensitive profiling of N-linked glycans both cleaved from chicken avidin and glycoproteins in human serum, indicating prospective availability for these labelling reagents in frontier of glycomics researches. (C) 2015 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21275009]; SCI(E); EI; PubMed; ARTICLE; zhouyl@pku.edu.cn; zxx@pku.edu.cn; 992-997; 144
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/415415]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhao, Ming-Zhe,Zhang, Yi-Wei,Yuan, Fang,et al. Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry[J]. TALANTA,2015.
APA	Zhao, Ming-Zhe.,Zhang, Yi-Wei.,Yuan, Fang.,Deng, Yan.,Liu, Jing-Xin.,...&Zhang, Xin-Xiang.(2015).Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry.TALANTA.
MLA	Zhao, Ming-Zhe,et al."Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry".TALANTA (2015).