EF-G catalyzes tRNA translocation by disrupting interactions between   decoding center and codon-anticodon duplex

doi:10.1038/nsmb.2869

CORC > 北京大学 > 化学与分子工程学院

	EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex
	Liu, Guangqiao ; Song, Guangtao ; Zhang, Danyang ; Zhang, Dejiu ; Li, Zhikai ; Lyu, Zhixin ; Dong, Jianshu ; Achenbach, John ; Gong, Weimin ; Zhao, Xin Sheng ; Nierhaus, Knud H. ; Qin, Yan
刊名	自然结构与分子生物学
	2014
关键词	ELONGATION-FACTOR-G PROTECTION PROTEIN TET(O) 30S RIBOSOMAL-SUBUNIT MESSENGER-RNA TETRACYCLINE RESISTANCE PRETRANSLOCATION STATE INTERMEDIATE STATES ESCHERICHIA-COLI STEM-LOOP P-SITES
DOI	10.1038/nsmb.2869
英文摘要	During translation, elongation factor G (EF-G) catalyzes the translocation of tRNA(2)-mRNA inside the ribosome. Trans location is coupled to a cycle of conformational rearrangements of the ribosomal machinery, and how EF-G initiates translocation remains unresolved. Here we performed systematic mutagenesis of Escherichia coli EF-G and analyzed inhibitory single-site mutants of EF-G that preserved pretranslocation (Pre)-state ribosomes with tRNAs in A/P and PIE sites (Pre-EF-G). Our results suggest that the interactions between the decoding center and the codon-anticodon duplex constitute the barrier for translocation. Catalysis of translocation by EF-G involves the factor's highly conserved loops I and II at the tip of domain IV, which disrupt the hydrogen bonds between the decoding center and the duplex to release the latter, hence inducing subsequent translocation events, namely 30S head swiveling and tRNA(2)-mRNA movement on the 30S subunit.; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); 0; ARTICLE; qiny@ibp.ac.cn; 9; 817-824; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/388336]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Guangqiao,Song, Guangtao,Zhang, Danyang,et al. EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex[J]. 自然结构与分子生物学,2014.
APA	Liu, Guangqiao.,Song, Guangtao.,Zhang, Danyang.,Zhang, Dejiu.,Li, Zhikai.,...&Qin, Yan.(2014).EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex.自然结构与分子生物学.
MLA	Liu, Guangqiao,et al."EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex".自然结构与分子生物学 (2014).