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	A study of anti-lipid peroxidation and injury protection effects of picroside II on hepatic cellular mitochondria
	Gao Hua ; Zhou Ya-wei
	2006
关键词	PARACETAMOL RAT HEPATOTOXICITY ACETAMINOPHEN TOXICITY
英文摘要	[Purpose] To study anti-lipid peroxidation and injury protection effects on hepatic cellular mitochondria in mice with liver damage. [Methods] Three animal models, with liver damage induced by carbon tetrachloride CCl4:0.1ml/10g, ip), D-galactosamine (D-GaIN: 500 mg/kg, ip) and acetaminophen (AP: 0.15g/kg, ip) respectively, were administered Picroside II at various levels of concentration (5, 10, 20 mg/kg, ig). The continuously monitoring method, recommended by International Clinical Chemistry League, was used to analyze serum levels of alanine aminotransferase,(ALT) and aspartate aminotransferase (AST), while Marland method was used to detect the activitiy of manganese-superoxide dismutase (SOD) in liver mitochondria. TBA colorimetry was employed to determine the content of malonic aldehyde (MDA) in liver tissue. The activity of glulathioneperoxidase (GSH-Px) was evaluated by DTNB method and the protein level in liver tissue was detected, by Lowry method. Meanwhile, protective effects of picroside If on the activity of ATPase and swollen extent of mitochondria in AP damaged hepatocytes were also evaluated. RESULTS: Picroside If showed significantly preventive effects on liver toxicity in the three models of liver damage. It decreased the high levels of ALT and AST in serum induced by the administration of CCl4, D GalN and AP, remarkably reduced livercellular damages, and appeared to. be even more potent than the positive control drug of biphenyl dimethyl dicarboxylate, pilules (DDB). In groups treated with different doses of Picroside II, compared to the model group, the content of MDA in serum decreased evidently; whereas the content of SOD and GSH-Px increased in a dose-dependent manner, and the difference was statistically significant. Further, the results of the AP model showed that picroside If could inhibit AP-induced liver toxicity, in mice, enhance the activity of ATPase, improve the swell extent of mitochondria, and help maintain a normal balance of energy metabolism.[Conclusion] Picroside If can protect hepatocyte injuries induced by CCl4, D-GaIN and AP. This protection effect may be achieved through the mechanism that Picroside II can help scavenge free radicals, protect normal constructions of mitochondria membrane and enhance the activitiy of ATPase in mitochondria, resulting in modulating the balance of liver energy metabolism. A Study of Anti-Lipid Peroxidation and Injury Protection Effects of Picroside II on Hepatic Cellular Mitochondria.; Public, Environmental & Occupational Health; CPCI-S(ISTP); 0
语种	英语
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/386145]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Gao Hua,Zhou Ya-wei. A study of anti-lipid peroxidation and injury protection effects of picroside II on hepatic cellular mitochondria. 2006-01-01.

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