Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine   in single living cells

doi:10.1111/j.1745-7254.2007.00545.x

CORC > 北京大学 > 化学与分子工程学院

	Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine in single living cells
	Xu, Ning ; Liang, Zhang-yi ; Xu, Ming ; Guan, Ying-hua ; He, Qi-hua ; Han, Qi-de ; Zhao, Xin-sheng ; Zhang, You-yi
刊名	中国药理学报
	2007
关键词	adrenergic receptor trajectory living cell ALPHA(1)-ADRENOCEPTOR SUBTYPES LOCALIZATION RECEPTOR DESENSITIZATION INFECTION
DOI	10.1111/j.1745-7254.2007.00545.x
英文摘要	Aim: To investigate the movement of alpha(1A)-adrenergic receptors(alpha(1A)-AR) stimulated by agonist, phenylephrine (PE), and the dynamics of receptor movement in real time in single living cells with millisecond resolution. Methods: We labeled alpha(1A)-AR using the monoclonal, anti-FLAG (a kind of tag) antibody and Cy3-conju-gated goat anti-mouse IgG and recorded the trajectory of their transport process in living HEK293A cells stimulated by agonist, PE, and then analyzed their dynamic properties. Results: The specific detection of alpha(1A)-AR on the surface of living HEK293A-alpha(1A) cells was achieved. alpha(1A)-AR internalize under the stimulation of PE. After the cells were stimulated with PE for 20 min, apparent colocalization was found between alpha(1A)-AR and F-actins. After 40 min stimulation of PE, trajectories of approximate linear motion in HEK293A-alpha(1A) cells were recorded, and their velocity was calculated. Conclusion: The specific labeling method on the living cell surface provides a convenient means of real-time detection of the behavior of surface receptors. By this method we were able to specifically detect alpha(1A)-AR and record the behavior of individual particles of receptors with 50 ms exposure time in real time in single living cells.; Chemistry, Multidisciplinary; Pharmacology & Pharmacy; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 1; ARTICLE; 6; 796-802; 28
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345573]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xu, Ning,Liang, Zhang-yi,Xu, Ming,et al. Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine in single living cells[J]. 中国药理学报,2007.
APA	Xu, Ning.,Liang, Zhang-yi.,Xu, Ming.,Guan, Ying-hua.,He, Qi-hua.,...&Zhang, You-yi.(2007).Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine in single living cells.中国药理学报.
MLA	Xu, Ning,et al."Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine in single living cells".中国药理学报 (2007).