Characterization of the Central Metabolic Pathways in Thermoanaerobacter sp. Strain X514 via Isotopomer-Assisted Metabolite Analysis

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	Characterization of the Central Metabolic Pathways in Thermoanaerobacter sp. Strain X514 via Isotopomer-Assisted Metabolite Analysis
	Feng Xueyang ; Mouttaki Housna ; Lin Lu ; Deng (等)
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2009
卷号	75 期号:15 页码:5001-5008
ISSN号	0099-2240
中文摘要	Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose or pyruvate as both carbon and energy sources. X514 grew on minimal medium and thus contains complete biosynthesis pathways for all macromolecule building blocks. Based on genome annotation and isotopic analysis of amino acids, three observations can be obtained about the central metabolic pathways in X514. First, the oxidative pentose phosphate pathway in X514 is not functional, and the tricarboxylic acid cycle is incomplete under fermentative growth conditions. Second, X514 contains (Re)-type citrate synthase activity, although no gene homologous to the recently characterized (Re)-type citrate synthase of Clostridium kluyveri was found. Third, the isoleucine in X514 is derived from acetyl coenzyme A and pyruvate via the citramalate pathway rather than being synthesized from threonine via threonine ammonia-lyase. The functionality of the citramalate synthase gene (cimA [Teth514_1204]) has been confirmed by enzymatic activity assays, while the presence of intracellular citramalate has been detected by mass spectrometry. This study demonstrates the merits of combining (13)C-assisted metabolite analysis, enzyme assays, and metabolite detection not only to examine genome sequence annotations but also to discover novel enzyme activities.
语种	英语
公开日期	2011-09-09
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn//handle/337004/534]
专题	青岛生物能源与过程研究所_单细胞中心
推荐引用方式 GB/T 7714	Feng Xueyang,Mouttaki Housna,Lin Lu,et al. Characterization of the Central Metabolic Pathways in Thermoanaerobacter sp. Strain X514 via Isotopomer-Assisted Metabolite Analysis[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(15):5001-5008.
APA	Feng Xueyang,Mouttaki Housna,Lin Lu,&等.(2009).Characterization of the Central Metabolic Pathways in Thermoanaerobacter sp. Strain X514 via Isotopomer-Assisted Metabolite Analysis.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(15),5001-5008.
MLA	Feng Xueyang,et al."Characterization of the Central Metabolic Pathways in Thermoanaerobacter sp. Strain X514 via Isotopomer-Assisted Metabolite Analysis".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.15(2009):5001-5008.